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All Journal Indian Journal of Forensic Medicine & Toxicology
Rohith Krishna
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Public Health

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Method Development and Optimization for Colchicine Estimation in Toxicological Analysis using Dispersive LiquidLiquid Micro-Extraction and HPLC-UV Rohith Krishna; Bhavana Shivankar
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14890

Abstract

Background: Colchicine is an alkaloid with anti-mitotic properties and has a narrow Therapeutic Indexwhich can be proven to be fatal at higher doses. The present study is an attempt to develop a method thatincludes an effective adaptation of Dispersive Liquid-Liquid Microextraction (DLLME) with optimizedparameters.Methods: The preliminary analysis was performed with UV-VIS Spectrophotometer. The analysis wasperformed using a Shimadzu LC-10AT with SPD- 10A UV-VIS Detector. The sample analyzed wasZycolchin 0.5mg. The developed method was validated in accordance with the ICH guidelines.Results: The LOD and LOQ was determined to be 6µg/ml and 19µg/ml respectively. The system suitabilitywas determined using the symmetry and efficiency calculation. The sample showed excellent linearity ofR2= 0.99. The present study facilitates an optimized methodology for extraction and analysis which ensuresgreat efficiency for the forensic analysis of colchicine. The study also highlights the importance of microextractions as they require only micro-level solvent usage which greatly improves the laboratory analysis.The entire extraction process is quick and effective for the analyte and is useful for the toxicological analysisin the forensic perspective.
Co-Authors Bhavana Shivankar