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All Journal Indian Journal of Forensic Medicine & Toxicology
Sahudi Abdul Mujib
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Public Health

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Increased Expression of Tubulin A, Cyclin B1, and Ki67 May Associate with Cell Death Mechanism Caused by Noncontact Electro Capacitive Cancer Exposure in vitro Sahudi Abdul Mujib
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14895

Abstract

Background: Noncontact Electro Capacitive Cancer Therapy (ECCT) is a novel treatment modality incancer that uses low-intensity intermediate-frequency alternating electric field. A previous study has reportedECCT could induce cell death in cancer cells, but the molecular mechanism underlining this process remainsunclear. Therefore, this study aims to uncover the molecular mechanisms of the pathology induced by thedevice and to determine whether Tubulin A, Cyclin B, and Ki67 can serve as biomarkers for this exposure.Methods: This study was an in vitro laboratory experiment with Completely Randomized Block Design.Two different types of cancerous cell lines were used in this study: oral squamous cell carcinomas and Helacells. In addition, non-cancerous cell line, bone marrow mesenchymal cell, was also subjected to the electricfield produced by ECCT. All three cell lines were divided into two groups: the ECCT-exposed group andthe control group. After exposure, the protein expression of Tubulin A, Cyclin B, and Ki-67 was examinedusing immunocytochemistry staining.Results: The results showed that the number of Tubulins A, Cyclin B1, and Ki-67 expression wassignificantly higher in ECCT-exposed group than in the control group (p<0.05). Increased expression oftubulin A indicates a disruption to microtubule polymerization, an increase in Cyclin B expression indicatesmitotic arrest or incomplete mitosis, whereas the increase of Ki-67 expression occurs when cells stop in themetaphase phase (mitotic arrest).Conclusion: It could be concluded that increased Tubulins A, Cyclin B1, and Ki-67 expression may associatewith the death cell mechanism induced by exposure to Noncontact Electro Capacitive Cancer devise.
Experimental Study of Collagen Density in Reconstruction of Tracheal Defect Using Dried Amniotic Membrane Sahudi Abdul Mujib; Sonny Seputra
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14896

Abstract

Background: Current tracheal defect management is still controversial for surgeons because there areno ideal materials to substitute trachea and many long-term complications occur in patients after trachealreconstruction. Prosthetic graft has the risks of chronic infection and post-reconstructive tracheal stenosis.Dried Amniotic Membrane (DAM) expresses several endogenous matrix metalloproteinase (MMP) genesthat can degrade extracellular matrix. Therefore, DAM is expected to be able to degrade collagen formedexcessively during the wound healing phase, hence post-reconstruction stenosis will not occur. This studyaimed to evaluate the differences of collagen density between polypropylene mesh (only) and DAM-addedpolypropylene mesh as graft in tracheal reconstruction.Methods: The animals used in this study were 36 male white rabbits, which were divided into twogroups. Both groups were wounded in the trachea area. The defects from the first group were closed usingpolypropylene mesh, while the defects from the second group were closed using DAM-added polypropylenemesh. After 30 days, the collagen density of each group was assessed.Results: In the first group there are 10 samples (55.56%) with collagen density score = 3, while in the secondgroup there are 16 samples (88.89%) with collagen density score = 2. The significance value using MannWhitney U test for differences in the collagen density score in both groups is p = 0.407.Conclusion: Tracheal reconstruction using polypropylene mesh along with DAM provides a lower collagendensity score than using polypropylene mesh alone as a graft, but it is not statistically significant.
Co-Authors Sonny Seputra