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All Journal Indian Journal of Forensic Medicine & Toxicology
Amit Jena
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Public Health

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A Questionnaire based Cross-Sectional Survey on PRF Monika Mohanty; Shashirekha Govind; Amit Jena; Naomi R Singh
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14973

Abstract

Progress in regenerative endodontic procedures needs approval by clinicians, but there is little or noevidence. This study aimed to examine the awareness, mindset and experience of regenerative endodonticsamong Indian dental residents. For the survey they used a self-administered questionnaire which consistedof three parts; part A focused on the background and experience of dentists, part B pursued the viewpoint,views and decision of the dentist about the utilize of PRF and Regenerative techniques, Whereas part Ccontained questions focused on their clinical experience. Findings indicate that maximum respondents knewabout PRF during postgraduate training but 90% are willing to attend PRF and REP training. However,few claimed to have used any form of regenerative therapy in their work. Finally, it can be concludedthat findings are an indication of optimistic dental residents concerning the use of regenerative endodonticmethods; however, further analysis and routine training is needed.
In Vitro Evaluation of Antimicrobial Efficacy of the Different Concentrations of Chitosan against Staphylococcus epidermidis in Disinfection of the Root Canal System Manali Nath; Shashirekha Govind; Amit Jena; Satabdi Pattanaik
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14977

Abstract

During endodontic procedures, along with mechanical instrumentation, irrigants are imperative for pathogeneradication. As the strains of pathogens are becoming antibiotic resistant at a constant ascending pace andalso the potentially harmful effect is associated with synthetic drugs, so studies on natural alternatives forirrigation are being done. Chitosan, a natural alternative, has been evaluated as an antimicrobial medicine.Recently, in cases presenting with persistent infections after endodontic treatment, it was noted that of all themicrobial species identified, the prevalence of s.epidermidis was the most. In this current study, an analysisof the antimicrobial effectiveness of chitosan as a potent root canal irrigant, for disinfecting the root canalwas conducted. Methodology: By the serial dilution method, the minimum inhibitory concentration wasdetermined. Dilution of the test solution was done using 10% DMSO solution. After which, incubationin a rotary incubator, at 180 rotations per minute, the temperature of 37?, for 24 hours was done. Forobtaining the zones of inhibition of different concentration (0.2%, 0.5%, 0.75, 1%) of chitosan irrigant, theagar diffusion method was applied and the resultant clear zone was measured with a ruler. Result: Chitosan(liquid) had promising antibacterial activity against Staphylococcus epidermidis, with a 16 mm zone ofinhibition at 100 µg/ml. Conclusion: Within the limitations of the present study, it was observed that, evenat lower concentrations, chitosan (liquid form) effectively hindered the further growth of S.epidermidis.
Co-Authors Manali Nath Monika Mohanty Naomi R Singh Satabdi Pattanaik Shashirekha Govind