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All Journal Jurnal Ilmu Pertanian Indonesia Seminar Nasional Teknik Kimia Kejuangan
Yoga Asmara
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Engineering Environmental Science

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Identifikasi Potensi Enzim Lipase dan Selulase pada Sampah Kulit Buah Hasil Fermentasi La Ode Sumarlin; Dikdik Mulyadi; . Suryatna; Yoga Asmara
Jurnal Ilmu Pertanian Indonesia Vol. 18 No. 3 (2013): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Fermentation is one of bioconversion to produce profitable anaerobic microbes and to produce various enzymes. Lipases and cellulases are widely used enzymes so far. Cellulases play an important role in bioconversion of organic waste cellulosic materials to glucose, single cell proteins, animal feed, and ethanol. Lipases can also degrade fatty ester bond. Therefore, both enzymes are potential to be used in industry as well as in households. Fermentation of fruit peel waste is an attempt to produce cellulase and lipase that can be carried out in a simple way. Cellulase as says was performed using DNS (3.5-dinitrosalicylic acid) and acid-base titration for analysis of lipase using cooking oil as the substrate. The results showed that the highest cellulase activity was obtained from watermelon rind mixed with citrus fruit peel of 0.036 U/mL, and mixed of banana peel and citrus fruit, which was 0.035 U/mL. The optimum lipase activity was at 30 oC, pH 7, and reaction time of 60 minutes. The highest lipase activity (1.36 U/mL) was obtained from mixture of watermelon and orange rind. Thus, the fruit peel waste is potential to produce cellulase and lipase by fermentation .
Produksi Gula Pereduksi dari Depolimerisasi Pati Singkong Melalui Proses Pelarutan disertai Pemanasan dan Hidrotermal Febriyati Puspasari; Yoga Asmara; Prida Novarita Trisanti; Sumarno .
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2017: PROSIDING SNTKK
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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In cassava, the major component are starch (60%). This component can be converted into a high valueproduct, such a reducing sugar and oligosaccharides. Starch conversion can be done by de-polymerizationusing a hydrothermal process. The effect of reaction time in hydrothermal process in reducing sugarproduction was studied in this research. Cassava starch suspension (1:20 (w/v)) was pre-mixing with heatingat 60°C and 45 minutes. Then proceed with the hydrothermal process at 100°C and 100 bar in variousreaction time (15-120 minutes). The solid product were analyzed using Scanning Electron Microscopy(SEM), X-Ray Diffraction (XRD), and Anthrone analysis (starch). And liquid products were analyzed by DNSanalysis to calculate concentration of reducing sugars. From SEM analysis showed hydrothermal processwas attacked the garnules surface deeply and also the inner grannules parts. And concentration of reducingsugars was increase by increasing hydrothermal time.
Co-Authors . Suryatna Dikdik Mulyadi Febriyati Puspasari La Ode Sumarlin Prida Novarita Trisanti Sumarno .