Ruth Chrisnasari
Fakultas Teknobiologi Universitas Surabaya

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EKSTRAK DAUN TEH HITAM SEBAGAI ANTIOKSIDAN ALAMI MINYAK KELAPA BLACK TEA LEAVES EXTRACT AS A NATURAL ANTIOXIDANT IN COCONUT OIL Agnes Belinda Ferina; Tjandra Pantjajani; Ruth Chrisnasari
CALYPTRA Vol. 7 No. 2 (2019): Calyptra : Jurnal Ilmiah Mahasiswa Universitas Surabaya (Maret)
Publisher : Perpustakaan Universitas Surabaya

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Abstract

Coconut oil can undergo rancidity during the usage. Rancidity in coconut oil can occur due to the oxidation process. Antioxidant mostly used to prevent oil oxidation. Synthetic antioxidant usually used as a preservative in commercial oil, however synthetic antioxidant had been known to cause several health problems. Therefore, this research was aimed to study the addition of antioxidant from crude extract of black tea leaves in coconut oil quality which treated to accelerate its rancidity. Black tea leaves were extracted using 3 different solvents to determine the best solvent to extract the antioxidant from black tea leaves which gave the highest antioxidant activity. The best solvent to extract the black tea leaves was found to be ethanol 96% with the antioxidant activity of 75,01%. As much as 0,01% (v/v) crude extract of black tea leaves then used for treatment to prevent the deterioration of coconut oil. The oil quality was assessed by measuring iodine value, peroxide value, TBA value and the change in colour and viscosity. The results showed that the addition of crude extract from black tea leaves delayed the oxidation rate in coconut oil compared to the control (without extract addition). The antioxidant efficiency of crude extract from black tea leaves was 1,5.
EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS) SEBAGAI XANTIOKSIDAN ALAMI MINYAK KELAPA (COCOS NUCIFERA) Yessyca Octavia; Ruth Chrisnasari; Maria Goretti Marianti P.
CALYPTRA Vol. 7 No. 2 (2019): Calyptra : Jurnal Ilmiah Mahasiswa Universitas Surabaya (Maret)
Publisher : Perpustakaan Universitas Surabaya

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Coconut oil can be used to fulfill the high demand for cooking oil in Indonesia. The quality of coconut oil can decrease because of oxidation. Oxidation can be prevented by adding natural antioxidant such as green tea. The objective of this research was to optimize the process of metabolite extraction with highest antioxidant activity. The solvent used for extraction were ethanol 96%, asetonitril 50% and acetone 25%. Green tea extract was analyzed quantitatively using Total Phenolic Compound (TPC) and 2,2-diphenyl-1-pichrylhydraziyl (DPPH). The crude extract from green tea from maceration using ethanol 96% gave the highest inhibition at about 90,8 ± 0,71%. Thin Layer Chromatography (TLC) analysis showed existence of catechin and myrecetin in the extract. As much as 0,01% was applied as natural antioxidant in coconut oil. The quality of coconut oil observed by iodine value, peroxide value, TBA value, colour and viscosity changed. The result showed that the addition of green tea extract can prevent oxidation rate with efficiency of 1,5.
FORMULASI PAKAN TERNAK SAPI PERAH BERBAHAN DASAR BLOTONG, TONGKOL JAGUNG, DAN TEPUNG IKAN KUALITAS RENDAH DENGAN PENAMBAHAN PROBIOTIK Euginius Eduard Effendi; Ruth Chrisnasari; Tjandra Pantjajani
CALYPTRA Vol. 7 No. 2 (2019): Calyptra : Jurnal Ilmiah Mahasiswa Universitas Surabaya (Maret)
Publisher : Perpustakaan Universitas Surabaya

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Cattle feed is a product made from a variety of organic compounds that are useful as a staple food of cattle. Filter cake usually called blotong is solid mud from the sugar mill industrial. Corn cob is an agriculture waste. Low quality fish flour is fish waste which is dried and milled. Cattle feed formulation made from raw materials has a completeness in meeting the nutritional needs of dairy cattle feed. The three ingredients processed into animal feed formulations to find the best and qualify the Indonesian National Standard (SNI). The best formulation with the composition of the filter cake 30%, 30% corn cobs, and low quality fish flour 40%. The formulation has a reduced sugar content of 0,21%, 1,6% total sugar, 4,23% water, 8,48% ash, 21,69% crude fiber, 21.3% protein, 1.84% fat, and pH of 6.39. Best cattle feed formulation was fermented by Lactobacillus acidophilusuntil seven days. After observation, reduced sugar content, total sugar, crude fiber, and pH were decreased. Probiotics was determined using Total Plate Count with the number of cells 6,12x1010 on the seventh day of fermentation.