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All Journal Jurnal Ilmu Pertanian Indonesia BIOGENESIS
Yeni Sari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Other

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Induksi Kalus Jeruk Kasturi (Citrus microcarpa) Menggunakan Hormon 2,4-D dan BAP dengan Metode in vitro Imam Mahadi; Wan Syafi’i; Yeni Sari
Jurnal Ilmu Pertanian Indonesia Vol. 21 No. 2 (2016): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.926 KB) | DOI: 10.18343/jipi.21.2.84

Abstract

Calamansi fruits (Citrus microcarpa) or musk lime is one horticulture crops has been known as beverage and food flavorings. This research aimed to determine the effect of 2,4-D and BAP on callus induction of explants calamansi fruits (Citrus microcarpa). Parameters observed is percentage of explants, time appeared callus, callus width, and texture of the callus. Research results and a growing percentage of the width callus explants were analyzed by ANOVA and a further test DMRT at 5% level. The next to appear callus timing parameters and texture of callus analyzed in description. The result shows that a combination of hormones D4B1 and D4B2 produces a callus emerged fastest of 3.3 days after culture. The highest percentage of callus formation that is 100% contained in the treatment of D2B0, D2B0.5-D4B0. The mean width of callus was highest in treatment D4B2with a width of 0.97 cm. Embryogenic callus resulting by treatment of D2B1, D2B2, and D3B0. However, the best a combination of hormones is treatment D2B2 (2 mg/l, 2,4-D, dan 2 mg/l BAP) producing embryogenic callus edvanced to suspension cultures.
PENGARUH PEMBERIAN HORMON 2,4-D DAN BAP TERHADAP PERTUMBUHAN KALUS JERUK KASTURI (Citrus microcarpa) Imam Mahadi; Wan Syafi'i; Yeni Sari
Biogenesis Vol 12, No 2 (2016)
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.036 KB) | DOI: 10.31258/biogenesis.12.2.97-101

Abstract

ABSTRACT The aims of this research to determine the effect of the hormone 2,4-D and BAP on callus growth Kasturi Orange (Citrus microcarpa).  This research consisted of research using experimental method completely randomized design factorial.  Parameters observed is percentage of explants, while appearing callus, callus width and texture of the callus. Research results and a growing percentage of the width callus explants were analyzed by ANOVA and a further test DMRT at 5% level, when it appears callus and callus texture done by descriptively. The result shows that a combination of hormones D4B1 and D4B2 produces a callus emerged fastest of 3.3 HSK. The highest percentage of callus formation that is 100% contained in the treatment of D2B0, D2B0.5 - D4B0. The mean width of callus was highest in treatment D4B2 with a width of 0.97cm. Embryogenic callus resulting by treatment of D2B1, D2B2 ,and D3B0.  Keywords: Hormones, Callus, Orange Kasturi            
Co-Authors Imam Mahadi Wan Syafi'i Wan Syafi’i