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All Journal Jurnal Ilmu Pertanian Indonesia AGRIVITA, Journal of Agricultural Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Iriawati Iriawati
Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Pengaruh Suhu Tinggi terhadap Perkembangan Organ Reproduksi Jantan pada Cabai (Capsicum annum L.) cv. Tanjung-2 Iriawati Iriawati; Isqim Oktaviani; Ahmad Faizal
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1297.61 KB) | DOI: 10.18343/jipi.25.1.19

Abstract

The development of male reproductive organ in plants is seriously affected by the adverse abiotic stresses. In this study, we investigated pollen development, particularly at microsporogenesis and micro gametogenesis stages of chili pepper (Capsicum annuum) cv. Tanjung-2 upon exposure with high temperature (day/night = 36/33°C). For this objective, anther of different flower sizes ranging from <2.5 mm; 3-4.5 mm; 4.5-7 mm; to 7-11 mm from treated and non-treated plants were collected. The results revealed that the early microsporogenesis stage was highly sensitive to high temperature indicated by a low progression of cells into subsequent process in meiotic division. This result was followed by the inhibition of tetrad formation up to 2%. Consequently, plant produced smaller microscpores and pollens with thin exin that resulted in the decreased pollen viability to 90%. In conclusion, high temperature has negative impact on the development of male reproductive program in chili pepper, including microsporogenesis and micro gametogenesis. Extending approach should be allocated to overcome this problem so that such environmental stress would not decrease the productivity of chili pepper. Keywords: Capsicum annuum, high temperature, microgametogenesis, microsporogenesis
Transient Transformation of Potato Plant (Solanum tuberosum L.) Granola Cultivar Using Syringe Agroinfiltration Yesy John Mba’u; Iriawati Iriawati; Ahmad Faizal
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1467

Abstract

Genetic transformation has been used as an alternative approach to improve the quality and the productivity of potato plant. In this study, different conditions have been set up to optimize transient GFP (Green Fluorescence Protein) expression in potato cv. Granola. Leaves of potato were infiltrated with Agrobacterium tumefaciens strain C58C1 harboring pK7FWGF2 vector with a nuclear-targeted GFP by simple pressure. GFP signals allowed simple evaluation of transformation efficiency which were indicated by GFP expression in nucleus of leaf cells in infiltratedareas. The results showed that leaf position, co-cultivation time, optical density and the presence of acetosyringone significantly affected the transformation efficiency. The fourth terminal leaves from four-week old plants were the optimum age for transformation. Furthermore, the highest transient transformation efficiency was obtained upon 48 h post infiltration at an OD600 = 0.8, and the presence of 200 μM acetosyringone. In conclusion, the developed protocol will be useful to study gene function as well as to generate stable transformation of this potato cultivar.
Respon Antera Lilium longiflorum Thunb. dengan Berbagai Stadium Perkembangan Mikrospora pada Kombinasi Konsentrasi Zat Pengatur Tumbuh Anggraeni Anggraeni; Iriawati Iriawati
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 1 No 2 (2017): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (856.513 KB) | DOI: 10.33019/agrosainstek.v1i2.7

Abstract

The right stage of microspore development to be used as explants become the critical factor for the successful of anther culture. Anther containing microspores at the pollen mother cell to binucleate stages were cultured on Murashige and Skoog (MS) media supplemented with various plant growth regulators. The media of 7.5 mM NAA + 0.75 mM BAP with bud size of 0.6-2.0 cm (pollen mother cell stage) are a combination that fits in callus, indirect shoot and direct shoot initiation with the percentage growth of each 30%; 16.6%; and 14.8%. Chromosome counts of root-tip cell of 89 regenerant revealed that 85 regenerant were diploids (95.5%) and 4 regenerant aneuploids (4.5%), but the haploid regenerants didn’t obtain. This result suggests that regenerants were derived from a somatic cells division.
Co-Authors Ahmad Faizal Anggraeni Anggraeni Isqim Oktaviani Yesy John Mba’u