Dono Wahyuno
Balai Penelitian Tanaman Rempah dan Obat, Bogor

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Puccinia xanthii Penyebab Bercak Daun pada Xanthium sp. di Indonesia Dono Wahyuno
Jurnal Fitopatologi Indonesia Vol 8 No 4 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.797 KB) | DOI: 10.14692/jfi.8.4.116

Abstract

An obligate plant parasitic rust fungi found on leaf of Xanthium sp. that grows wildly in Raihat County, Belu District of East Nusa Tenggara Province. Morphological observation of the fungal structure under compound light microscope revealed the fungus belongs to Puccinia. Spore is pedicelate, the pedicles were hyaline, 10,0-(20,9)-30.0 μm long and 3.8-(4.9)-7.5 μm wide; the teliospores were orange to light brown color, 22.5-43.9)-55.0 μm long, and 15.0-(17.3) 20.0 μm wide; the apical thickening wall present at the spore tip, 3.8-(6.6)-10.0 μm thick and has microcyclic life cycle type. The fungus was identified as Puccinia xanthii and this is the first report the occurrence of the fungus in Indonesia.Key words: leaf spot, Puccinia xanthii, Xanthium
Pengaruh Kemasaman, Suhu, dan Cahaya terhadap Golovinomyces sordidus Penyebab Penyakit Embun Tepung pada Plantago major Dini Florina; Dyah Manohara; Dono Wahyuno
Jurnal Fitopatologi Indonesia Vol 10 No 5 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.639 KB) | DOI: 10.14692/jfi.10.5.170

Abstract

Unidentified powdery mildew was found on leaves of a medicinal plant, Plantago major in Indonesia. The present studies were aimed to identify the causal fungal species of powdery mildew of  P. major and study the effect of acidity, temperature and light on the causal fungus. Identification was conducted by observing morphological characteristics of the fungus scraped from diseased leaves under light microscope. The infection process was observed by staining the inoculated leaves followed observation under light microscope. Conidia were suspended in various pH solutions to examine effect of pH on conidial germination and hyphal length. For testing effects of temperature on conidial germination and hyphal length, conidial suspensions were dropped onto glass slides then incubated in temperature ranges from 20–35 °C, the germinating conidia and length of the existing hypha were counted and measured 24 hours later. With the same method as above the glass slides were incubated in 25 °C either in the dark or exposed under illuminated white light tube (400 Lux) for study effect of light on conidial germination. The causal fungus was identified as Golovinomyces sordidus (syn. Erysiphe sordida) with its anamorph state as Oidium. The germinating conidia penetrate directly into leaf tissue within 24 hours. The optimal conditions for the conidia to germinate are pH between 4 and 7, temperature between 25 °C and 30 °C, and dark condition.