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Endang Nurhayati
Institut Pertanian Bogor
Medicine & Pharmacology

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Virulensi Isolat Rice tungro virus dari Beberapa Daerah Endemis Tungro di Indonesia Suprihanto Suprihanto; Endang Nurhayati; Jumanto Harjosudarmo
Jurnal Fitopatologi Indonesia Vol 9 No 1 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (595.615 KB) | DOI: 10.14692/jfi.9.1.29

Abstract

The research was conducted to distinguish the virulence of Rice tungro virus (RTV) isolates from several tungro disease endemic areas in Indonesia based on their response in differential cultivars of rice. Eight isolates of RTV were collected from several tungro endemic regions in Indonesia i.e. Bali (Denpasar), South Kalimantan (Tanah Laut), South Sulawesi (Lanrang), West Java (Subang and Bogor), Central Java (Solo), East Java (Jember), and West Nusa Tenggara (Mataram). The isolates were maintained on TN1 rice cultivar in greenhouse by successive transfers via Nephotettix virescens. Each isolate was subsequently passed on to FK135 and TN1 rice cultivars. Four week after inoculation, plants responses were observed to compare their symptom and infectivity on the differential cultivars. The results showed that the level of virulence of RTV isolate from tungro disease endemic areas in Indonesia can be classified into 3 levels. The highest virulence were RTV isolates from West Jawa, the moderate level of virulence were RTV isolates from East Java, South Kalimantan, South Sulawesi, and West Nusa Tenggara, the low level of virulence were RTV isolates from Bali and Central Java.Key words: differential cultivar, Nephotettix virescens, rice, tungro diseases
Efisiensi Tular Benih Squash mosaic virus pada Cucurbitaceae Susanti Mugi Lestari; Endang Nurhayati
Jurnal Fitopatologi Indonesia Vol 10 No 3 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.701 KB) | DOI: 10.14692/jfi.10.3.81

Abstract

Infection of viruses on Cucurbitaceae may cause high yield and economic losses. Squash mosaic virus is a seed borne virus and among the most important virus infecting Cucurbitaceae. The aims of these research was to detect infection of several viruses on Cucurbitaceae and to examine seed transmission efficiency of SqMV. Detection of Cucumber mosaic virus (CMV), Squash mosaic virus (SqMV), Watermelon mosaic virus-2 (WMV-2), Zucchini yellow mosaic virus (ZYMV), and Tobacco ringspot virus (TRSV) from field samples and seeds was conducted using Indirect-ELISA method. Infection of CMV, SqMV and ZYMV was detected from field samples. Seed transmission of SqMV on commercial seeds of bottle gourd, watermelon, zucchini, cabocha, cucumber, and melon was 13, 13, 33, 73, 100, and 100%, respectively. Seed transmission of ZYMV was only occurred on bottle gourd and zucchini, i.e. 13.3% and 26.67%, respectively. Infection of SqMV through F2 seed was determined from cucumber, bottle gourd, and melon, i.e. 93, 100, and 100%, respectively. Therefore, the status of SqMV as quarantine pest should be evaluated since SqMV was already found in West Java.
Co-Authors Jumanto Harjosudarmo Suprihanto Suprihanto Susanti Mugi Lestari