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All Journal Jurnal Fitopatologi Indonesia AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian

Meity Suradji Sinaga

Institut Pertanian Bogor

Author-ID : 2452240

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Medicine & Pharmacology

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Articles

Identifikasi Penyebab Penyakit Busuk Pangkal Batang pada Jeruk Eka Retnosari; Julinda Bendalina Dengga Henuk; Meity Suradji Sinaga
Jurnal Fitopatologi Indonesia Vol 10 No 3 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

One major disease of citrus is basal stem rot which may cause significant constraint on citrus production in Indonesia. Research was initiated to identify the causal agent of basal stem rot disease from 11 citrus growing areas in Indonesia. Identification of fungal pathogens was based on macroscopic and microscopic observation of morphological characters. Koch Postulate was conducted to confirm the causal agent of the disease. Two fungal pathogens, Botryodiplodia theobromae, and Phytophthora citrophthora were found associated with basal stem rot disease. B. theobromaewere isolated from all plant samples, whereas P. citrophthorawas only isolated on samples from Soe (East Nusa Tenggara). Characteristic symptoms of basal stem rot disease was developed on plants inoculated with B. theobromae and P. citrophthora; this confirmed that two fungal isolates was the causal agent of basal stem rot disease.

Perlakuan Udara Panas untuk Pengendalian Perkecambahan Spora Tilletia indica pada Gandum Nurul Dwi Handayani; Taufan Tanto Setyawan; Salbiah Salbiah; Dono Wahyuno; Meity Suradji Sinaga
Jurnal Fitopatologi Indonesia Vol. 14 No. 1 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Karnal bunt of wheat (Triticum aestivum) caused by Tilletia indica is a major concern in international trade because it can degrade quality significantly, and it is a quarantine pest for Indonesia. Tilletia infected seeds can be the source of fungal inoculum of other areas. The current research was aimed to determine the effectiveness of temperature and exposure time to control Tilletia using air heat treatment without reducing the quality of grain. The experiments were conducted in vitro, initiated by seed testing, including morphology-based detection and identification of Tilletia followed by a viability test of the seed. Air heat treatment was performed by adjusting the oven temperature at 75, 80, 85 °C and 28 °C (control) for 4 and 6 hr exposure time, each with three replications. The results showed that air heat treatment of the wheat at 75 °C for 4 hr was able to suppress teliospores germination of 56.7–61.7%, without denature protein content of the wheat.

Eksplorasi dan Karakterisasi Khamir dan Bakteri sebagai Agens Antagonis terhadap Penyebab Penyakit Blas pada Padi Jauharoh Maknunah; Meity Suradji Sinaga
Jurnal Fitopatologi Indonesia Vol. 14 No. 3 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Exploration of Yeasts and Bacteria as Antagonist Agent of Rice Blast PathogenBlast disease (Pyricularia oryzae) is a major disease of rice that causes 61% yield losses. An alternative way to control the disease is applying antagonistic agents, both yeasts and bacteria. This study was aimed to obtain yeast and bacterial isolates that can be used as antagonistic agents to effectively control blast disease. Yeasts and bacteria were isolated from the leaves of Digitaria sp., Panicum sp., and Pennisetum sp.. The isolates of yeasts and bacteria were screened following hypersensitive test on tobacco leaves, direct planting method test on ‘Ciherang’ rice seed, and hemolysis test on blood agar medium. The isolates were also further assayed for their antagonism through dual culture test, volatile compound test, hyperparasitism test, and chitinolytic activity test. Three isolates of yeast, i.e. Cryptococcus sp., Rhodotorula sp., and Candida sp., and two bacterial isolates, i.e. Bacillus sp. and isolate PPY were identified as potential antagonists of P. oryzae.

Trichoderma dan Gliocladium untuk Mengendalikan Penyakit Busuk Akar Fusarium pada Bibit Kelapa Sawit Siti Juariyah; Efi Toding Tondok; Meity Suradji Sinaga
Jurnal Fitopatologi Indonesia Vol 14 No 6 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Trichoderma and Gliocladium for Controling Fusarium Root Rot Disease of Oil Palm SeedlingsFusarium spp. have been reported as the causal agent of common spear rot and crown rot diseases on oil palm. An effective strategy to control these diseases is not available yet. This research was aimed to find biocontrol agents for effective control of crown rot disease on oil palm seedlings caused by Fusarium spp. The experiment consisted of 3 parts i.e. pathogenicity test of 3 isolates of Fusarium, identification and in vitro test of biocontrol agents, and in planta test of biocontrol agents against Fusarium spp. In vitro test was done through dual culture test and test for volatile compound produced by the biocontrol agents. In planta test was conducted through inoculation of Fusarium spp. into oil palm seedlings growing on medium containing selected biocontrol agents i.e. Trichoderma harzianum, T. virens, T. inhamatum, and Gliocladium fimbriatum. In vitro test showed that Gliocladium fimbriatum 1 and 2 were inhibited effectively the growth of Fusarium spp. on the dual culture test, whereas T. harzianum Gadingrejo 2 was inhibited effectively the growth of Fusarium spp. on volatile compound test. The application of biocontrol agents was effective to protect oil palm seedlings from Fusarium spp. infection.

Potensi dan Mekanisme Yeast-Like Fungus Pseudozyma dalam Mengendalikan Antraknosa pada Cabai Sri Hartati; Suryo Wiyono; Sri Hendrastuti Hidayat; Meity Suradji Sinaga
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 7 No 1 (2023): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v7i1.275

Application of biocontrol agents on pre- and post-harvest commodities is a promising disease control strategy. This research was objected to study the potencies and antagonism mechanisms of eight isolates of yeast-like fungus Pseudozyma, i.e. P. hubeiensis Dmg 18 BEP, Dmg 20 DEP, Dmg 23 DEP, Dmg 27 BEP, and Dmg 32 DEP, P. shanxiensis Dmg 28 DEP, dan P. aphidis SG 25 BE and SG 53 BE in controlling Colletotrichum acutatum, the cause of anthracnose on chili. Those isolates of Pseudozyma were obtain from chili leaf and fruit surfaces and tissues. To study their potencies in controlling anthracnose on chili caused by C. acutatum, the eight Pseudozyma isolates were applied on chili fruit. While the study of the antagonism mechanisms was performed by antibiosis, volatile compound formation, chitinolitic activities, hyperparasitism, and ACC deaminase production tests. The results showed that all isolates of the Pseudozyma had the potencies to control anthracnose on chili caused by C. acutatum with more than 60% control levels. The mechanisms were production of volatile compounds, chitinolitic activities, and hyperparasitism. Those Pseudozyma isolates did not produce ACC deaminase.

Co-Authors Dono Wahyuno Efi Toding Tondok Eka Retnosari Hermanu Triwidodo Jauharoh Maknunah Julinda Bendalina Dengga Henuk Nurul Dwi Handayani Salbiah Salbiah Siti Juariyah Sri Hartati SRI HENDRASTUTI HIDAYAT Suryo Wiyono Taufan Tanto Setyawan Yundari, Yundari

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