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All Journal Jurnal Fitopatologi Indonesia
Ummu Salamah Rustiani
Balai Uji Terap Teknik dan Metode Karantina , Pertanian, Bekasi
Medicine & Pharmacology

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Deteksi Bakteri Penyebab CVPD pada Jeruk Menggunakan DNA Asal Tulang Daun Ummu Salamah Rustiani; Ariningsih Salji Endah; Nurjanah Nurjanah; Andi Prasetiawan; Nurmaida Nurmaida
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.252 KB) | DOI: 10.14692/jfi.11.3.79

Abstract

A method for identification of the causal bacteria of citrus vein phloem degeneration (CVPD) based on polymerase chain reaction (PCR) technique using template DNA extracted from leaf midrib of citrus has been implemented routinely. The method has not been validated, therefore it is necessary to validate the method to confirm that the method fit for its intended use. Leaf samples showing chlorotic symptom was obtained from Bogor and Bekasi, West Java and used for test samples. These samples was differentiated into 2 groups, i.e. leaf midrib and leaf mesophyll. Validation test involved homogenicity, and reproducibility test; each test was replicated 2 times. The test showed that using leaf midrib gave better result for detection of bacteria causing CVPD disease than using leaf mesophyll. Therefore, this method is recommended as routine detection method for bacteria causing CVPD disease.
Co-Authors Andi Prasetiawan Ariningsih Salji Endah Nurjanah Nurjanah Nurmaida Nurmaida