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Kemampuan Campuran Bacillus sp., Pseudomonas fluorescens, dan Trichoderma sp. untuk Mengendalikan Penyakit Layu Bakteri pada Tanaman Tomat abdul manan; Endang Mugiastuti; Loekas Soesanto
Jurnal Fitopatologi Indonesia Vol. 14 No. 2 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Ability of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. to Control Tomato Bacterial Wilt DiseaseRalstonia solanacearum is known as the causal agent of bacterial wilt on tomato. The bacteria may infect all stadia of plant growth and decrease tomato production. Biological control using antagonistic microbes is considered as a potential control alternative for the disease. This research was aimed to assay the ability of combination treatment of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. in controlling R. solanacearum and its effect on growth and yield of tomato in the field. The treatments consisted of control (without antagonistic microbes), mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp.; Bacillus sp. B8 + Pseudomonas flourescens P8 + Trichoderma sp.; and Streptomisin sulfat 20%. Antagonistic microbes was applied at planting time, as much as 100 mL per plant. The result showed that mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp. was the best treatment in controlling the disease as indicated by delaying incubation period up to 6.2 days, decreasing disease incidence up to 12.3%, increasing plant growth up to 42.80%, and increasing yield up to 14.99%.

SOSIALISASI DAN APLIKASI METABOLIT SEKUNDER MIKROBA ANTAGONIS UNTUK MENGENDALIKAN PENYAKIT DAN MENINGKATKAN HASIL TANAMAN JAGUNG DI KECAMATAN SUMBANG KABUPATEN BANYUMAS JAWA TENGAH Loekas Soesanto; Abdul Manan; Endang Mugiastuti
Jurnal Abditani Vol. 2 No. 2 (2019): Oktober
Publisher : FAKULTAS PERTANIAN UNIVERSITAS ALKHAIRAAT

Kelompok tani “Mugi Rahayu” Desa Tambaksogra, Kecamatan Sumbang Kabupaten Banyumas Jawa Tengah merupakan kelompok tani yang banyak mengusahakan tanaman jagung. Permasalahan utama yang dihadapi dalam peningkatan produksinya adalah tingginya serangan penyakit tanaman. Sosialisasi pengelolaan penyakit jagung dengan menggunakan metabolit sekunder mikroba antagonis perlu dilakukan. Tujuan kegiatan adalah meningkatkan pengetahuan dan keterampilan petani tentang penyakit tanaman jagung serta tindakan pengelolaannya dengan metabolit sekunder. Permasalahan mitra diatasi dengan beberapa inovasi teknologi, yang dilakukan dengan metode transfer teknologi melalui pendidikan, pelatihan, demplot, dan pendampingan. Kegiatan sosialisasi mampu meningkatkan pengetahuan petani tentang jenis penyakit jagung, pengendaliannya, dan pengendalian yang ramah lingkungan sebesar 72,98%. Sedangkan. kegiatan pelatihan perbanyakan dan metabolit sekunder mampu meningkatkan kemampuan petani masing-masing sebesar 48,57 % dan 34,09 %. Pertumbuhan dan hasil jagung pada petak metabolit sekunder setara dibandingkan dengan petak petani dengan menggunakan pestisida kimia sintetik

Penerapan Teknologi Pengendalian Penyakit Tanaman Yang Ramah Lingkungan Pada Tembakau Endang Mugiastuti; Loekas Soesanto; Abdul Manan
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 2 NOMOR 2 SEPTEMBER 2018 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Tobacco is one of the most important plants which were cultivated by Farmers Groups "Darma Mukti Tani I" and "Karya Tani" of Wangon District, Banyumas Regency. However, several obstacles often interfere with the production of tobacco, including quite high tobacco pests and diseases. Socialization of plant pests diseases management by using Trichoderma sp. and Pseudomonas fluorescens biopesticides, needs to be done. The activity aimed was to increase knowledge and skills of farmers on pests and diseases in tobacco and their management measures with biological pesticides. Issues addressed farmers partners with several technology solution, which was conducted by the transfer of technology through education, training, demonstration plots, and mentoring. Dissemination activities were able to increase their knowledge of pests and diseases, their control, and the control of environmental friendly as 58,82, 62,5, and 67.67%, respectively. The training activities of P. fluorescens exploration was able to improve their ability as 166.67%. Propagation activity and application training could improve their ability for 100 and 73,33%, respectively. The training activities of Trichoderma sp. exploration were able to improve their ability as 94,44%, while the propagation and application training could improve their ability, respectively, as 73,33 and 88.23%. Application of biological pesticides P. fluorescens and Trichoderma sp. at demonstration plot was able to increase growth and reduced the intensity of bacterial and fusarium wilt disease by 40-50%.Keywords: Tobacco, technology transfer, pests and diseases, biological control.

Pelatihan Teknologi Budidaya dan Pengelolaan Penyakit Ramah Lingkungan Pada Tanaman Lada Endang Mugiastuti; Loekas Soesanto; Abdul Manan
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 8, No 2 (2018): Desember 2018
Publisher : LPPM UNINUS

Pepper is an important commodity and has high economic value. This commodity has begun to be cultivated by members of the PKK RT 07 and PKK RT 09, Tambak Sogra Village, Sumbang District, Banyumas Regency, in an effort to increase the use of the yard and increase family income. However, the knowledge and skills of PKK members regarding cultivation and diseases of pepper plants as well as effective and environmentally friendly management efforts are very low. The activity aimed was to improve knowledge and skills of members of the PKK group in the cultivation and management of pepper disease that are effective and environmentally friendly. Activities carried out with dissemination, training, and technology demonstration plots. The activities were able to increase the knowledge and skills of members of the PKK group in pepper cultivation and the management of the disease which is environmentally friendly. In addition, the application of Trichoderma sp. and Pseudomonas fluorescens in the demonstration plot can increase the growth of pepper.

Sosialisasi dan Pelatihan Pemanfaatan Biopestisida Mikroba Antagonis Campuran untuk Mengendalikan Penyakit Bawang Merah di Musim Hujan Abdul Manan; Endang Mugiastuti; Loekas Soesanto
Jurnal Pengabdian Pada Masyarakat Vol 6 No 1 (2021)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202161.630

SOCIALIZATION AND TRAINING ON THE UTILIZATION OF MIXED ANTAGONISTIC MICROBIAL BIOPESTICIDES TO CONTROL SHALLOTS IN THE RAINY SEASON. The constraints on shallot cultivation in the rainy season are the more severe attacks of Fusarium oxysporum (Fusarium wilt) and Alternaria porii (purple disease). Solid formula of Trichoderma sp., Bacillus sp. B8, and Bacillus sp. B11 have been proven to increase their effectiveness and also cultivation technique and environmental modification. However, the benefit of the technology did not informed members of Farmers Group in Tambaksogra Village, Sumbang Regency, Banyumas District yet. The objectives of the activity are to increase farmers’ knowledge and skills on shallot cultivation technique out of the season, to multiply and to apply the antagonistic microbes solid mixed formula, to increase the crop production quantity and quality, and to increase farmers’ income. The method used was transfer of the technology through education, training, and demplot. The activities carried out were socialization of the potency and application of shallot cultivation technique out the season, multiplication and application of the antagonistic microbial solid mixed formula with the use of available media at the location, demonstration plot of the shallot cultivation technology with the application of the solid mixed formulation compared to single antagonistic, and farmers’ accompaniment in implementing the technology package. Result showed that there was an increase in farmers’ knowledge on the potency and application of solid formula biopesticides by 69.61%, as well as an increase skill in making solid formula biopesticide and its application by 64.33%.

Pengelolaan Tanaman Bawang Merah Ramah Lingkungan dengan Pemanfaatan Biopestisida Trichoderma Abdul Manan; Nurtiati Nurtiati; Endang Mugiastuti
Jurnal SOLMA Vol. 7 No. 2 (2018)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29405/solma.v7i2.2160

Kelompok tani "Rukun Tani I” Desa Sumbang , Kecamatan Sumbang Kabupaten Banyumas Jawa Tengah merupakan kelompok tani yang banyak mengusahakan tanaman hortikultur. Permasalahan utama yang dihadapi dalam peningkatan produksi bawang merah adalah tingginya serangan penyakit moler (Fusarium oxysporum) dan hama ulat bawang (Spodoptera exigua). Sosialisasi pengelolaan hama dan penyakit bawang merah dengan menggunakan biopestisida Trichoderma sp. perlu dilakukan. Tujuan kegiatan adalah meningkatkan pengetahuan dan keterampilan petani tentang hama dan penyakit bawang merah serta tindakan pengelolaannya dengan pestisida hayati berbasis Trichoderma. Permasalahan mitra diatasi dengan beberapa pemecahan teknologi, yang dilakukan dengan metode transfer teknologi melalui pendidikan, pelatihan, demplot, dan pendampingan. Kegiatan sosialisasi mampu meningkatkan pengetahuan petani tentang jenis hama dan penyakit bawang merah, pengendaliannya, dan pengendalian yang ramah lingkungan sebesar 72,98%. Sedangkan. kegiatan pelatihan perbanyakan dan aplikasi Trichoderma mampu meningkatkan kemampuan petani masing-masing sebesar 48,57 % dan 34,09 %. Aplikasi pestisida hayati Trichoderma sp. pada petak demplot mampu meningkatkan pertumbuhan dan hasil bawang merah sebesar 7,09 % dan menurunkan intensitas hama dan penyakit sebesar 34,16 %.

Raw Secondary Metabolites of Trichoderma harzianum T10 in Tapioca Flour Towards Cucumber Damping-off Loekas Soesanto; Hidayatul Ilahiyyah; Endang Mugiastuti; Abdul Manan; Rostaman Rostaman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24567

Trichoderma harzianum is effective for controlling soil-borne pathogenic fungi and producing secondary metabolites. When applied in the field, the raw secondary metabolites are quickly decreased directly by sunlight. One strategy to avoid degradation is the use of tapioca fluor liquid formula for biological control agents. This research aimed to obtain the most effective concentration of tapioca flour in development of raw secondary metabolites of Trichoderma harzianum T10, its effect on damping-off and growth of cucumber. This research was carried out at the screen house and the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University from September 2017 up to January 2018. The study was conducted in two stages, i.e., in vitro and in planta. The in vitro stage used completely randomized design with five repetitions and five treatments consisted of T. harzianum T10 in Potato Dextrose Broth, and in 0.5; 1; 1.5; and 2% of tapioca flour media. In in planta, randomized block design was used with five repetitions and six treatments consisted of control, T. harzianum T10 in PDB, and in 0.5; 1; 1.5, and 2% of tapioca flour media. Variables observed were density of conidia, disease incubation period, disease incidence, AUDPC, maximum growth potential, germination ability, plant height, canopy fresh weight, root length, and fresh root weight. Result of the research showed that the highest conidial density (1.23 x 107 conidia mL-1) of T. harzianum T10 was found in 2% tapioca flour with an increase of 63.28% compared to the PDB. The tapioca flour of 1 and 2%, and PDB could suppress the disease incidence by 81.82%. The lowest AUDPC was at 2% tapioca flour. The raw secondary metabolites could not delay the incubation period significantly and increase cucumber plant growth.Â The novelty is the use of antagonistic fungi in terms of raw secondary metabolites and the discovery of tapioca flour with the right concentration to produce high conidia density and high raw secondary metabolites. TheÂ benefits are to find other cheaper ingredients in promoting antagonistic fungal growth and the use of antagonistic fungal bioactive compounds to control plant pathogen

Pengelolaan Tanaman Bawang Merah Ramah Lingkungan dengan Pemanfaatan Biopestisida Trichoderma Abdul Manan; Nurtiati Nurtiati; Endang Mugiastuti
Jurnal SOLMA Vol. 7 No. 2 (2018)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29405/solma.v7i2.2160

Kelompok tani "Rukun Tani I” Desa Sumbang , Kecamatan Sumbang Kabupaten Banyumas Jawa Tengah merupakan kelompok tani yang banyak mengusahakan tanaman hortikultur. Permasalahan utama yang dihadapi dalam peningkatan produksi bawang merah adalah tingginya serangan penyakit moler (Fusarium oxysporum) dan hama ulat bawang (Spodoptera exigua). Sosialisasi pengelolaan hama dan penyakit bawang merah dengan menggunakan biopestisida Trichoderma sp. perlu dilakukan. Tujuan kegiatan adalah meningkatkan pengetahuan dan keterampilan petani tentang hama dan penyakit bawang merah serta tindakan pengelolaannya dengan pestisida hayati berbasis Trichoderma. Permasalahan mitra diatasi dengan beberapa pemecahan teknologi, yang dilakukan dengan metode transfer teknologi melalui pendidikan, pelatihan, demplot, dan pendampingan. Kegiatan sosialisasi mampu meningkatkan pengetahuan petani tentang jenis hama dan penyakit bawang merah, pengendaliannya, dan pengendalian yang ramah lingkungan sebesar 72,98%. Sedangkan. kegiatan pelatihan perbanyakan dan aplikasi Trichoderma mampu meningkatkan kemampuan petani masing-masing sebesar 48,57 % dan 34,09 %. Aplikasi pestisida hayati Trichoderma sp. pada petak demplot mampu meningkatkan pertumbuhan dan hasil bawang merah sebesar 7,09 % dan menurunkan intensitas hama dan penyakit sebesar 34,16 %.

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