Samarah Jafar Alwash
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Detection of toxin-associated genes in seven spa-types of Staphylococcus aureus in Iraq Samarah Jafar Alwash; Rasmiya Abed Aburesha
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15945

Abstract

Background: Staphylococci frequently cause opportunistic infections in patients with underlying disease, such as those with prosthetic devices, surgical patients, and patients with diabetes or individuals undergoing dialysis. Consequently, detecting the toxin-associated genes in different S. aureus strains, isolated from patients with wound and burn injuries and their environments, is essential to monitor and prevent the complication in such cases.Methods: Identification ofS. aureus that isolated from burn and wound injuries has been done according to the biochemical tests, then Spa-typing method was used to diagnose at the strain level. Toxin-associated genes, enterotoxin gene(sea, seb),exfoliative gene(eta), toxic shock syndrome gene(tst), and pantonevalentine leukocidin(pvl), were detected by polymerase chain reaction (PCR) method.Results: Two isolates were harboring tstand a single isolate harbored pvl, at the time that no isolates were foundetaandsebpositive among 19 S.aureus. While sea was the predominant toxin-associate gene in the studied S. aureus strains, it was detected in 13 isolates.Conclusion: Distribution of specific spa-type/sea-positive in diabetic foot ulcers at hospitals, indicating the poor sanitization conditions at Iraqi wound wards, and it is recommended to take swabs from the patients and their environments as a routine procedure
Antimicrobial Effect of Bacillus Spp. on MRSA Samarah Jafar Alwash; Zahraa A. Jabur; Maha Mohammad; Saad Sabah Fakhry; Farqad Farhan Abdulhammed
Journal of Global Pharma Technology Vol. 9 Issue 7 : 2017
Publisher : Journal of Global Pharma Technology

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Abstract

There are many antimicrobial peptides produced by Bacillus species, which have a bacteriostatic and/or bactericidal activity. The aim of this study is to investigate the antimicrobial activity of Bacillus spp. against methicillin-resistant Staphylococcus aureus (MRSA) by analyzing 59 Bacillus spp. isolated from rice and spices using the agar well diffusion method in terms of their general inhibition effects on some MRSA isolates. Nine isolates were found to be effective against MRSA and their effects were compared with some antibiotics. The study showed that many Bacillus spp. has antimicrobial effect against MRSA3 which is resistant to all antibiotics except vancomycin. It has been found that Bacillus lentus is more effective against MRSA when compared with some antibiotics. Further studies may be required to assess the use of Bacillus lentus as therapeutic and bio preservative in food processing industry.Keywords: MRSA, Bacillus lentus, Antimicrobial peptides, Antibiotic, API-CHB-50.