Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Jurnal Teknologi Dan Industri Pangan
J. F. Laksmi
PAU Bioteknologi IPB Jl. Puspa 1 Institut Pertanian Bogor, Bogor 16610
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
STUDI PENDAHULUAN ENZIM KITINASE EXTRASELULER YANG DIHASILKAN OLEH ISOLAT BAKTERI ASAL MANADO 1) [Preliminary Study of Extracellular Chitinase Produced by Bacteria Isolated from Manado] E. Y. Purwani; A. Toharisman; E. Chasanah; J. F. Laksmi; V. Welan; M. T. Suhartono; T. Purwadaria; J. K. Hwang; Y. R. Pyun
Jurnal Teknologi dan Industri Pangan Vol. 13 No. 2 (2002): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.125 KB)

Abstract

Chitinolytic bacteria were isolated from several exotic area in Manado Province. The most potential isolate, namely 13.26, was isolated from Tompaso. The isolate was cultured in the thermus medium containing colloidal chitin as a carbon source for 5 days at 55°C to produce chitinase. It was observed that chitinase was most active at 65°C and the optimum pH was 8 in boric acid-borax buffer. Ammonium sulfate (50% saturation) precipitation of the protein increased the specific activity of the enzyme from 0.20 unit/mg protein (in culture supernatant) to 0.28 unit/mg protein. The molecular weight as estimated by zymogram analysis was180 kDa.
Co-Authors A. Toharisman E. Chasanah E. Y. Purwani J. K. Hwang M. T. Suhartono T. Purwadaria V. Welan Y. R. Pyun