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All Journal Jurnal Teknologi Dan Industri Pangan
Betty S. L. Jenie
Departemen Ilmu dan Teknologi Pangan, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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AKTIVITAS ANTIBAKTERI FRAKSI-FRAKSI EKSTRAK SIRIH HIJAU (Piper betle Linn) TERHADAP PATOGEN PANGAN [Antibacterial Activity of Fractionated Green Sirih (Piper betle Linn) Extract Against Food Pathogenic Bacteria] . Suliantari; Betty S. L. Jenie; Maggy T. Suhartono
Jurnal Teknologi dan Industri Pangan Vol. 23 No. 2 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.882 KB) | DOI: 10.6066/jtip.2012.23.2.217

Abstract

Fractionation of green sirih (Piper betle Linn) extract by chromatography colom using the mixture of several solvents i.e. chloroform, ethanol and acetic acid (4:1:1) resulted in 17 fractions. All fractions showed antibacterial activities but only 2 fractions (fraction 3 and fraction 4) showed the highest inhibition towards the six tested bacteria Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes. Among the tested bacteria, all fractions of green sirih extracts showed the most effective inhibition against, Salmonella Typhimurium with inhibition zone diameters ranging from 10 mm to 26 mm. Identification using GC-MS found that fraction 3 and fraction 4 contained chavicol; dodecanoic acid, myristic, palmitic and oleic acid.  
PENGARUH RETROGRADASI DAN PERLAKUAN KELEMBABAN PANAS TERHADAP KADAR PATI RESISTEN TIPE III DALUGA Agustina -; Didah Nur Faridah; Betty S. L. Jenie
Jurnal Teknologi dan Industri Pangan Vol. 27 No. 1 (2016): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.953 KB) | DOI: 10.6066/jtip.2016.27.1.78

Abstract

The giant swamp taro starch or daluga corm starch in this study was extracted from corms collected from Siau Island of North Sulawesi Province in Indonesia. Native starch daluga corm has low resistant starch content, so starch modifications method were needed to increase its resistant starch content. The effect of acid hydrolisis, debranching, autoclaving-cooling and heat moisture treatment on the changes of RS3 daluga were studied. The corrm starch was first modified with acid hydrolysis (HA) and debranching with pullulanase (D). The starch was then modified with one cycle autoclaving-cooling (HADAC1x), HADAC1x with heat moisture treatment (HADAC1x+HMT), three cycle autoclaving-cooling (HADAC3x), HADAC3x with heat moisture treatment (HADAC3x+HMT) which increased its RS3 content to 42.37, 40.47, 31.12, and 31.18% (db), respectively. The starch modification method can affect other chemical properties changes such as starch content, amylose content, and reducing sugar content.
PEMURNIAN DAN KARAKTERISASI PEDIOSIN YANG DIPRODUKSI OLEH Pediococcus pentosaceus 2A2 Ni Wayan Triwulandhari; Betty S. L. Jenie; Hanifah N. Lioe; Irma Isnafia Arief
Jurnal Teknologi dan Industri Pangan Vol. 30 No. 2 (2019): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.999 KB) | DOI: 10.6066/jtip.2019.30.2.91

Abstract

Pediocin production by Pediococcus pentosaceus 2A2 was done in 30% molasses based medium at 37°C for 24 hours. The culture was then centrifuged to separate the cells and yield cell-free supernatant (CFS). Purification of the CFS was carried out by stepwise ammonium sulfate precipitation to achieve a 90% concentrate, followed by dialysis using membrane with a molecular weight cut off 2.0 kDa resulting in pediocin crude extract (PCE). The PCE was purified by cation exchange chromatography using SP Se-phadex C-25 and eluted with sodium acetat buffer at pH of 5.5; 6.0; 6.5; and 7.0 successively resulting in 4 fractions (F1, F2, F3, F4). Each purification step resulted in the increase in antimicrobial activity against Listeria monocytogenes ATCC 7644. The PCE had specific antimicrobial activity up to 5 fold higher than the CFS. F4 fraction, which showed the largest inhibition zone among the other fractions, had a very high specific activity up to 435 fold higher than the CFS. The SDS-PAGE analysis suggested that the molecular weight of F4 was approximately 5.9 kDa. The minimum inhibitory concentration (MIC) of F4 to inhibit 1 log (90%) of L. monocytogenes ATCC 7644 was 143 ppm. The inhibitory phenomenon observed under scanning electron microscope (SEM) showed that L. monocytogenes’s cells exposed to F4 experience morphological changes such as cells shrinkage.
Co-Authors . Suliantari Agustina - Hanifah Nuryani Lioe Irma Isnafia Arief Maggy T. Suhartono Ni Wayan Triwulandhari