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All Journal Indian Journal of Forensic Medicine & Toxicology Journal of Global Pharma Technology
Ali Kadhim Aljarah
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Medicine & Pharmacology Public Health

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Invasion of Diabetic and Atherosclerotic Vessels Patients with Periopathogen Spirochete Treponema denticola in Iraqi Population AL-Nasrawi; Adil Ateyah Abd Ali; Ali Kadhim Aljarah; Hayder Ali Muhammed
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.16219

Abstract

Both Periodontitis & Atherosclerosis of Cardiovascular system are chronic inflammatory disorders can beperformed by invasion of their connective tissues with aerobic & anaerobic infectious bacteria & formationof polymicrobial biofilms. Microbial & metabolic plaques are considered to be the initiating factor for manyinfectious & metabolic diseases, invasion of an oral & systemic organs with various opportunistic pathogenshave been reported in many populations. Several studies have been published Conventional & Real timePCR with 16S r RNA gene to improve the existence of the DNA molecules of the most invasive periodonticspathogens in atheromatous plaques of diabetic & cardiovascular blood vessels disease patients.In the present study, among 100 atherosclerotic & diabetic patients suffered from various degrees of ischemicheart disease, the DNA molecules of Treponema denticola was detected in (45/100)45% of atheromatousplaque samples significantly in male 28 /45 (62%) more than 17/45(38%) in female patients (p = 0.07) withno significant importance in cardiovascular & diabetic patients more than 50 years old 23/45(51%) whichwas approximate to 22/45(49%) in younger than 50 years old. As well as, T. denticola DNA molecules weresignificantly displayed in atheromatous plaques of type (1, 2) diabetic patients 35 /45 (78%) more than innon-diabetic group 10/45(22%) (p= 0.07) & T. denticola DNA molecules did not displayed in the controllergroup.In conclusion: the present cross sectional study demonstrated the invasion of atheromatous plaques ofcardiovascular atherosclerotic vessels & diabetic patients with highly aggressive anaerobic periodontalspirochetes T. denticola, these results improve the existence of additional risk factor that interfere withthe pathogenesis of periodontitis & genesis of cardiovascular atherosclerosis & may be correlated with theatherosclerosis & diabetes mellitus diseases patients in Iraqi population.
Genetic Detection for Staphylococcus aureus bacterialin Urinary Tract Infection cases in Hilla City Ali Kadhim Aljarah
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
Publisher : Journal of Global Pharma Technology

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Abstract

Fourteen urine Specimens were collected of patient with Different Ages & gender that had been infected with the UTI that previously Diagnosed in Hilla Educational Hospital in period from Feb. 2016 to Mar. 2016 for detection of Bacterial causative agent that cause the UTI , samples were transferred directly to laboratory then centrifuged in ( 3000 rpm ) for 15 min., taking pellet for bacterial culturing on mannitol salt agar in 37C° for 24 hr. in aerobic conditions changing the media to yellow color as an indicator for growth of S.aureusthen followed by DNA isolation from bacteria for further genetic detection by PCR, selecting ( mec A1 gene ) as an indicator gene that related to the virulence factors in the S.aureus, agarose gel electrophoresis for  mec A1 primer amplification showed 32 positive result indicated to the presence of this bacteria as one of most causative bacterial pathogenic agent in UTI, these rapid definitive diagnosis is useful for further treatment by selection the determined antibiotic drug latterly.Keywords: Mec A gene, Staphylococcus aureus, UTI, PCR
Co-Authors Adil Ateyah Abd Ali AL-Nasrawi Hayder Ali Muhammed