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All Journal Indian Journal of Forensic Medicine & Toxicology Journal of Global Pharma Technology
Jawad K. Abood Al-Janabi
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Medicine & Pharmacology Public Health

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A novel Screening Methods using SSCP –PCR to Detect Variations Among three Varieties of Trigonella Foenum Graecum L. Collected from Different Regions in Iraq Zahraa Abid Nima Al-Yasiry; Jawad K. Abood Al-Janabi; Basheer Al-Alwani
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.16259

Abstract

Fenugreek is one of the most commonly used herbal medicinal plants. Another benefit of fenugreek seedsis that it is used to enhance the flavor, color and texture of fruit. Fenugrek comprises a number of chemicalcomponents such as trigonellines, choline, flavonoids and fixed oils. SSCP –PCR is useful to reach rare andendemic species’ genetic diversity and also to resolve genetic links between populations. . PCR amplificationof DNA. Genotyping of ITS4 was performed using a polymerase chain reaction technique, followed by singlestrandconformation polymorphism. Accordingly, these DNA polymorphisms were confirmed using DNAsequencing. The results appeared that the presence of three different haplotype patterns named accordingto the number of bands were 4-bands, 3-bands, 2 –bands using SSCP –PCR technique. The pattern of allresolved SSCP bands can however be difficult to establish using gel visualisation alone. This polymorphismsof DNA must then be verified by the use of DNA sequencing. The findings of the sequence shown that thehaplotypes of the ITS region have been identified by several SNPs.Conclusion: our findings indicated that SSCP-PCR technique is more informative for evaluation of geneticdiversity and relationships among fenugreek populations.
Phenotypic and Molecular Identification of Trichophyton Rubrum and Microsporum gypseum of Dermatophytosis Jawad K. Abood Al-Janabi
Journal of Global Pharma Technology Volume 9 Issue 10
Publisher : Journal of Global Pharma Technology

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Abstract

Introduction: Dermatophytes fungi are the predominant source of skin, hair, and nails infection. The present study was carried out to identify and characterize the species of dermatophytes. Material and Methods: Predicated upon the convectional methods such as morphological and microscopic features, culture and biochemical techniques along with growth comparison on Sabouraud dextrose agar (SDA). Two species of fungi belonging to genera Trichophyton and Microsporum were culled to pursue ITS predicated PCR amplification of their genetic profile.  Results: The results of PCR amplification have shown that the ITS region, including 5.8S was successfully amplified by utilizing ITS1 and ITS4 as a primers. The amplicon size of dermatophytes species appeared in between 680-720bp, four isolates of T. rubrum and one isolate of M. gypseum were recorded in various regions of the patients bodies. Other fungi (non dermatophytes) such as Geotrichium candidaus, Candida albicans, Pencillium nutatum, Alternaria alternata, Aspergilus niger and Fusarium sp. were also observed on the infected human body with amplicon size in the range of 350-590 bp. Conclusion: These results have suggested not only a valuable baseline to identify pathogenic fungi but additionally emphasizes on future efforts toward the obviation of dermatophytic infections in our region.Keywords:  Identification, Trichophyton, Macrosporu   m, Dermatophyte mycosis.
Co-Authors Basheer Al-Alwani Zahraa Abid Nima Al-Yasiry