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All Journal Jurnal Hortikultura Indonesia (JHI) Plumula : Berkala Ilmiah Agroteknologi Agroista: Jurnal Agroteknologi
Siti Subandiyah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Energy Immunology & microbiology Materials Science & Nanotechnology

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Molecular and Biochemical Detection of Fusarium Oxysporum f.sp. cubense as the Pathogen of Fusarium Wilt Disease on Banana (Musa spp.) , Masanto; , Parwito; Arif Wibowo; Siti Subandiyah
Jurnal Hortikultura Indonesia Vol. 1 No. 1 (2010): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (986.06 KB) | DOI: 10.29244/jhi.1.1.40-45

Abstract

ABSTRACTMolecular and biochemical characteristics of Fusarium oxysporum f.sp. cubense (Foc) were detected. Six of Indonesian Foc isolates were artificially inoculated on “Ambon Kuning” banana. DNA of one-week culture isolates was extracted by three methods prior to PCR assay using Foc TR4 (tropical race 4) specific primer. Activity of extracellular enzyme was determined with reduction sugar, agar diffusion and SDS-PAGE assays. Statistical analysis revealed that all isolates insignificantly caused Fusarium wilt symptoms on tested banana with disease severity index ranging from 3 to 3.6. Maximum DNA concentration was obtained by CTAB method (766.25 µg mL-1), followed by SDS and alkaline l ysis methods, i.e. 553.75 and 211.25 µg mL-1, respectively. PCR analysis showed that Bnt2 and Kjg1 isolates positively reacted to TR4 of Foc primer (DNA size of 1400 bp approximately). Reduction sugar and agar diffusion assays demonstrated that Kjg1 isolate significantly produced more extracellular enzyme, with 6.53 × 10-2mg mL-1 in conce ntration and 20 mm in halo diameter. Meanwhile, SDS-PAGE assay viewed diverse bands of tested fungi (20.6 to 80 kDa), representing four extracellular enzymes. Positive PCR results highlighted the presence of Foc TR4 infecting banana in Indonesia. Various activities of extracellular enzymes did not influence the pathogenicity of Foc.Key words: pathogenicity, DNA concentration, extracellular enzyme
KADAR HARA PADA PENAMBAHAN PUPUK KANDANG DAN SILIKA PADA TANAH SUPRESIF DAN KONDUSIF LAYU FUSARIUM PADA PISANG Medina Uli Alba Somala; Sri Nuryani Hidayah Utami; Arif Wibowo; Siti Subandiyah
Plumula : Berkala Ilmiah Agroteknologi Vol 6 No 2 (2018): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.277 KB) | DOI: 10.33005/plumula.v6i2.7

Abstract

Fusarium wilt in bananas is caused by Fusarium oxysporum f.sp. cubense (Foc). Foc is one of the soil-borne pathogens. The intensity of disease attacks in different conditions with very severe is conducive soil. Suppressive soil can suppress and control the rate of development of soil-borne pathogens. The aims of this study are to determine the physical and chemical properties of soil and environment against fusarium wilt and find out the effects and levels of nutrients from the addition of manure and silica to fusarium banana wilt. Supressive soil samples from Sarampad, Cianjur and conducive soil from Salaman, Magelang. Ambon Kuning banana cultivar derived from tissue culture were planted to find out effects of manure and silica against fusarium wilt. Suppressive soil has higher soil chemist including neutral pH, sandy loam of texture class, total N, total P, organic C, organic matter, available Si than conducive soil. Manure and silica addition have significantly suppressed fusarium wilt on suppressive soil. The final experiment suppressive soil has higher total N, total K, organic C and organic matter than conducive soil.
DETEKSI KOMUNITAS BAKTERI PADA DIAPHORINA CITRI JANTAN DAN BETINA, VEKTOR PENYAKIT HUANGLONGBING PADA JERUK MENGGUNAKAN PCR-RISA Achmad Himawan; Siti Subandiyah
AGROISTA : Jurnal Agroteknologi Vol. 1 No. 1 (2017): MEI
Publisher : Program Studi Agroteknologi INSTIPER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (234.755 KB) | DOI: 10.55180/agi.v1i1.5

Abstract

Huanglongbing (HLB) is a very destructive disease on citrus. Diaphorina citri is a HLB disease vector. The aim of this research is to detec bacterial community in the insect vector, especially D. citri males and females, using Polimerase Chain Reaction-Ribosomal Intergenic Spacer Analysis (PCR-RISA) method. The results of research show that there are 3 – 4 bacteria species in D. citri males and 1 – 4 bacteria species in D. citri females.
Co-Authors , Masanto , Parwito Achmad Himawan Arif Wibowo Medina Uli Alba Somala, Medina Uli Alba Sri Nuryani Hidayah Utami