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Front Cover JBBI Vol 6, No 1, June 2019 Catur Sriherwanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (885.549 KB) | DOI: 10.29122/jbbi.v6i1.3704

Abstract

MUTATION OF LIPASE-PRODUCING BACTERIAL ISOLATE FROM PALM OIL EFFLUENT FOR FAT HYDROLYSIS ON POME dadang suhendar; Ika Rahmatul Layly; Gabriela Christy Sabbathini; Deden Rosid Waltam; Edi Wahjono; Catur Sriherwanto; Haniyya Haniyya
Jurnal Bioteknologi dan Biosains Indonesia Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Lipase is applied to hydrolyzethe residual oil or fat in palm oil mill effluent (POME), which serves as a feedstock for biogas production. A bacterial isolate (Bacillus velezensis) exhibiting lipase activity, previously obtained from Malimping, Pandeglang, Banten, Indonesia, underwentsequential treatments of gamma irradiation (1, 2, and 3 kGy), EMS (ethyl methane sulfonate) mutagenesis (0.5% v/v), and NTG (N-methyl N-nitrosoguanidine) (1 mg mL-1). Following each mutation, lipase activities of the mutant colonies were measured and compared to the wild-type strain. The results revealed that all mutated B. velezensiscolonies exhibited lipase activity values (7.78 ± 0.80 –9.05 ± 0.23 U mL-1) that were not significantly different from the wild-type isolate (8.31 ± 0.01 U mL-1), indicating strong bacterial resistance against the mutagenic treatments. The crude lipase preparation effectively hydrolyzed POME, resulting in a reduction of the COD value (from 131,450 ppm to 88,450 ppm) and O&G content (from 41,400 mg L-1to 5,770 mg L-1) within a 72-hour timeframe.
INHIBITORY ACTIVITY OF Trichoderma harzianum AGAINST PUTATIVELY PATHOGENIC FUNGUS ON RODENT TUBER (Typhonium flagelliforme) PLANT Catur Sriherwanto
Jurnal Bioteknologi dan Biosains Indonesia Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Trichoderma spp. are globally considered as the most dominant biofungicide in the market. Reports on Trichoderma spp. efficacy against pathogenic fungi in commercial crops have been numerous, but much less in medicinal plants. This study aimed at testing the potential biofungicidal activity of Trichoderma harzianum in inhibiting the growth of a putatively pathogenic fungus isolated from rodent tuber (Typhonium flagelliforme) plant. The methods consisted of isolation of fungi from the plant’s surface, soil, and polybags. The isolates were then screened for their putative pathogenicity against rodent tuber before being subjected to 16S rRNA molecular identification and in vitro antagonist test using T. harzianum. Result showed that only isolate K4 showed pathogenicity on T. flagelliforme, and was molecularly identified as Lasiodiplodia theobromae, known globally as fungal pathogen attacking various plants. L. theobromae was inhibited by T. harzianum with inhibition index of 23.0 ± 4.3%, which was about twice higher than that of the positive control nystatin 100.000 IU mL (11.1 ± 0.6%). In conclusion, T. harzianum inhibited the growth of L. theobromae in vitro, hence indicating its biofungicidal potential.