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Sajuthi D
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Effect of glycerol and dimethylformamide cryoprotectants on buck Etawah Crossbreed frozen semen using modified tris diluents. OS, Ariantie; TL, Yusuf; D, Sajuthi; RI, Arifiantini
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 4 (2013)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.833 KB) | DOI: 10.14334/jitv.v18i4.327

Abstract

A cryoprotectan is component that must be present in a cryopreservation medium to minimize the physical and chemical stresses resulting from the cooling, freezing and thawing of sperm cells. This study was carried out to determine the effect of glycerol (G) and dimethylformamide (DMF) as cryprotective agent in tris-egg yolk (TEY) trehalose (T) and tris-soya (TS) raffinose (R) diluents. Semen were collected from three sexually mature bucks using artificial vagina, evaluated and divided into four aliquot. Each of them was diluted with TEY suplemented with 50 mM trehalose and TS supplemented with 50 mM raffinose, added with glycerol or DMF 4% (v/v). Diluted semen was packed in minitube straw (100 x 106 sperm/0.25 mL) and equilibrated for 4 hours at 5°C, then freeze in N2 vapor for 10 minutes in styrofoam box and stored in liquid N2 container (-196) until futher evaluation. Progressive motility, viability and plasma membrane intact were evaluated after thawed at 37°C for 30 seconds factorial experimental design (2 x 2) was used in this study. The sperm motility in TEYTG was significantly higher (65.07±5.38%) compared to TEYTDMF (61.67±5.55%). In contrast sperm diluted with TSRDMF indicated better motility (42.22±8.13%) than TSRG (39.07±5.38%). It was concluded that cryoprotectant had different effect on different diluents. Key Words: Buck Etawah Crossbreed, Cryoprotectant, Diluent, Frozen Semen
Carcass profile and chemical characteristic of male Kacang goat (Capra aegragus hircus) I, Mirdhayati; J, Hermanianto; CH, Wijaya; D, Sajuthi
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.143 KB) | DOI: 10.14334/jitv.v19i1.991

Abstract

The purpose of this research was to characterize the carcass profile and chemical characteristic of male Kacang goat (local name for Capra aegragus hircus) from two different slaughter ages, namely < 1.5 and > 1.5 years old. Kacang goats generally is slaughtered for consumption at these ages. The body part used for chemical analysis was the carcass leg. Data was analyzed statistically by t test. Results showed that there was no profile differences of the carcass from both group ages obtained from similar rearing system and at common range of body weight for slaughtering. Chemical characteristics measured by the contents of protein, fat, moisture, ash and cholesterol level in goat meat showed no differences between the two slaughter ages. Dominant amino acids found in the goat meat was glutamic acid, aspartic acid, leucyne and lysine. Saturated and unsaturated fatty acid profiles in the goat meat did not show any different between the two slaughter ages, except for eicosatrienoic acid, in which the meat from the younger goat had significantly higher content of eicosatrienoic acid. The meat of male Kacang goat of both groups is a potential source of healthy meat, based on its low fat content, functional amino acid composition, higher desirable fatty acids and fatty acid ratio of omega 6 : omega 3 which corresponds to the values recommended by American Heart Association 2008. Key Words: Male Kacang Goat (Capra aegragus hircus), Carcass, Cholesterol, Amino Acids, Fatty Acids
Effect of glycerol and dimethylformamide cryoprotectants on buck Etawah Crossbreed frozen semen using modified tris diluents. Ariantie OS; Yusuf TL; Sajuthi D; Arifiantini RI
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 4 (2013): DECEMBER 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.833 KB) | DOI: 10.14334/jitv.v18i4.327

Abstract

A cryoprotectan is component that must be present in a cryopreservation medium to minimize the physical and chemical stresses resulting from the cooling, freezing and thawing of sperm cells. This study was carried out to determine the effect of glycerol (G) and dimethylformamide (DMF) as cryprotective agent in tris-egg yolk (TEY) trehalose (T) and tris-soya (TS) raffinose (R) diluents. Semen were collected from three sexually mature bucks using artificial vagina, evaluated and divided into four aliquot. Each of them was diluted with TEY suplemented with 50 mM trehalose and TS supplemented with 50 mM raffinose, added with glycerol or DMF 4% (v/v). Diluted semen was packed in minitube straw (100 x 106 sperm/0.25 mL) and equilibrated for 4 hours at 5°C, then freeze in N2 vapor for 10 minutes in styrofoam box and stored in liquid N2 container (-196) until futher evaluation. Progressive motility, viability and plasma membrane intact were evaluated after thawed at 37°C for 30 seconds factorial experimental design (2 x 2) was used in this study. The sperm motility in TEYTG was significantly higher (65.07±5.38%) compared to TEYTDMF (61.67±5.55%). In contrast sperm diluted with TSRDMF indicated better motility (42.22±8.13%) than TSRG (39.07±5.38%). It was concluded that cryoprotectant had different effect on different diluents. Key Words: Buck Etawah Crossbreed, Cryoprotectant, Diluent, Frozen Semen
Carcass profile and chemical characteristic of male Kacang goat (Capra aegragus hircus) Mirdhayati I; Hermanianto J; Wijaya CH; Sajuthi D
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.143 KB) | DOI: 10.14334/jitv.v19i1.991

Abstract

The purpose of this research was to characterize the carcass profile and chemical characteristic of male Kacang goat (local name for Capra aegragus hircus) from two different slaughter ages, namely < 1.5 and > 1.5 years old. Kacang goats generally is slaughtered for consumption at these ages. The body part used for chemical analysis was the carcass leg. Data was analyzed statistically by t test. Results showed that there was no profile differences of the carcass from both group ages obtained from similar rearing system and at common range of body weight for slaughtering. Chemical characteristics measured by the contents of protein, fat, moisture, ash and cholesterol level in goat meat showed no differences between the two slaughter ages. Dominant amino acids found in the goat meat was glutamic acid, aspartic acid, leucyne and lysine. Saturated and unsaturated fatty acid profiles in the goat meat did not show any different between the two slaughter ages, except for eicosatrienoic acid, in which the meat from the younger goat had significantly higher content of eicosatrienoic acid. The meat of male Kacang goat of both groups is a potential source of healthy meat, based on its low fat content, functional amino acid composition, higher desirable fatty acids and fatty acid ratio of omega 6 : omega 3 which corresponds to the values recommended by American Heart Association 2008. Key Words: Male Kacang Goat (Capra aegragus hircus), Carcass, Cholesterol, Amino Acids, Fatty Acids
Co-Authors Ariantie OS Ariantie OS Arifiantini RI Hermanianto J Mirdhayati I Wijaya CH Yusuf TL