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Ridad Agoes
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Medicine & Pharmacology

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Vegetables contamination by Parasitic Helminth Eggs in Malaysia and Indonesia Reashnaa Loganathan; Ridad Agoes; Insi Farisa Desy Arya
Althea Medical Journal Vol 3, No 2 (2016)
Publisher : Faculty of Medicine Universitas Padjadjaran

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Background: Soil-transmitted helminth infection is known to be a serious issue in South East Asia when the farmers use night soil and contaminated water for their plants. In Indonesia, some of the farmers still use human feces and sometimes also mixed it with urine from the latrines as fertilizers. On the contrary, in Malaysia these contamination occured at a lower rate due to strict rules by the authorities.The objective of this study was to identify the helminth eggs in vegetables from traditional markets in Indonesia and Malaysia.Methods: Three traditional markets were selected by non-random sampling, namely Pasar Ampang in Malaysia and 2 traditional markets in Indonesia, i.e Pasar Tanjungsari and Jatinangor. Cabbages and lettuce were bought from 15 different vegetable sellers per market. The samples were examined at the laboratory of the Medical Faculty, Universitas Padjadjaran. The method to obtain the specimen was in accordance with Khairul Anwar and Ramachandran. This study was carried out from July to October 2014. The collected data was analyzed by percentage and frequency tabulation.Result: Most of the samples are contaminated by helminth eggs. In Pasar Ampang, 13.3% in cabbage and 6.7% in lettuce. In Pasar Tanjung Sari, 46.7% in cabbage and 40% in lettuce. Moreover, in Pasar Jatinangor, 33.3% in cabbage and 26.7% in lettuce. Most of the helmint eggs were Ascaris lumbricoides.Conclusions: The helminthes eggs contamination is higher in Indonesian traditional markets compared to in Malaysia. [AMJ.2016;3(2):190–4]DOI: 10.15850/amj.v3n2.796
Identification of Pathogen Protozoans (Cryptosporidium spp and Giardia lamblia) from Canteen’s Water-sources at Jatinangor, West Java, Indonesia Komathi Nair Murugesan; Ridad Agoes; Sri Yusnita Irda Sari
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Faculty of Medicine Universitas Padjadjaran

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Background: The prevalence of intestinal protozoan infection caused by Cryptosporidium spp and Giardia lamblia has been rapidly increasing in developing countries. Contamination of water-sources by intestinal protozoans can spread infection to humans when it is consumed without any specific treatment. Jatinangor is an educational area where many universities are located and many students live in this area. This study aimed to identify the presence of intestinal protozoa (Cryptosporidium spp and Giardia lamblia) in water-sources used by canteens at Jatinangor area to detect the magnitude of protozoans intestinal infections.Methods: A descriptive cross sectional study was carried out from August to September 2014. Raw water samples from reservoir tanks, water containers and tapwater were taken from all canteens located along the main road of Jatinangor. Samples were centrifuged and divided into two portions; two drops from the first test tube was stained with Lugol solution and observed by 2 observers under a light microscope for Giardia lamblia, and sediments from the second test tube was stained using acid fast staining then observed for Cryptosporidium spp under the light microscope. Results: Out of 50 samples, 26 were positive for parasites, of which 22 (44%) were contaminated by Cryptosporidium spp and 4 (8%) were contaminated by Giardia lamblia.Conclusions: Half of the raw water-sources in Jatinangor are contaminated by Cryptosporidium spp and Giardia lamblia. Proper water treatment should be implemented by the owners of the canteens before it is consumed as drinking water. 
Co-Authors Insi Farisa Desy Arya Komathi Nair Murugesan Reashnaa Loganathan Sri Yusnita Irda Sari