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All Journal Medical Laboratory Technology Journal Tropical Health and Medical Research Journal Jurnal Praba: Jurnal Rumpun Kesehatan Umum
Utama, Ricko Dharmadi
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Religion Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Manual and Automated Comparatıve Analysıs in Retıculocyte Countıng Method Ashim Manta; Nitumani Khaklari; Gayatri Gogoi; Ricko Dharmadi Utama; Ahmad Muhlisin
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.002 KB) | DOI: 10.31964/mltj.v6i2.409

Abstract

The traditional method of reticulocyte count is based on microscopic evaluation of peripheral blood films. Automated processes have replaced the manual method of reticulocyte counts in most laboratories. They are readily available and give more precise results. The present study was conducted to compare the manual and automated methods of reticulocyte estimation. The study was conducted for six months, from January to June 2019, at a tertiary care center in Dibrugarh, Assam. One hundred twenty-six venous blood samples of patients were analyzed, of whom 27 were males and 99 females. Student t-test between the two methods showed a p-value of 0.41. Pearson's coefficient between the two methods showed r value =0.992. Scatter plot and Passing &Bablock regression analysis showed a positive correlation between the two methods. The present study showed a good correlation between manual and automated methods. The manual method can use the manual method of counting reticulocytes in situations of limited cost. In contrast, the automatic method can be an option when fast results and large sample sizes are required.
Analysis of Adolescent Hemoglobin Levels on Knowledge, Body Mass Index, and Menstrual Patterns Siti Mas'odah; Ratih Dewi Dwiyanti; Ricko Dharmadi Utama
Tropical Health and Medical Research Vol. 3 No. 2 (2021): Tropical Health and Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.11 KB) | DOI: 10.35916/thmr.v3i2.55

Abstract

Anemia can be related to several factors, including knowledge, body mass index, and menstrual patterns. Adolescent girls are a high-risk group who experience anemia. This study aims to analyze the hemoglobin levels of adolescent girls about knowledge, body mass index, and patterns menstrual. Information on the relationship between hemoglobin levels and these factors expects to be a reference for strategies to overcome anemia in adolescent girls. The sample of this research is the students of Martapura Indonesia vocational high school, totaling 45 people. Data collection includes weight and height measurements, hemoglobin examination (using Easy Touch GCHb), and questionnaires. Data analysis used bivariate analysis of chi-square test with 0.05 with 95% confidence degree. Results Respondents had fewer hemoglobin levels 47%, normal 53%. The body mass index value of respondents is less than 85%, normal 13%, excess 2%. The respondent's menstrual pattern obtained 26% abnormal data, 74% normal data. Knowledge of anemia of respondents with enough category 34%, good category 66%. The conclusion is that there is no relationship between hemoglobin levels and body mass index (p-value 0.281), menstrual patterns (p-value 0.073), and knowledge of anemia (p-value 0.402) in adolescent girls. Hemoglobin levels may more influence by the consumption pattern of foods containing iron, so it recommends that young women consume more iron-rich foods and avoid the simultaneous consumption of substances that inhibit their absorption, such as tea and coffee.
Media Lokal Ekonomis Untuk Kultivasi Trichophyton Rubrum: Inovasi Kacang Hijau, Glukosa Dan Kitin Aima Insana; Siti Rachmah; Ricko Dharmadi Utama
Jurnal Praba : Jurnal Rumpun Kesehatan Umum Vol. 3 No. 3 (2025): September : Jurnal Praba : Jurnal Rumpun Kesehatan Umum
Publisher : STIKES Columbia Asia Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62027/praba.v3i3.571

Abstract

This study examined the effect of modified mung bean powder (Vigna radiata) media with the addition of glucose and chitin on the growth of Trichophyton rubrum. The results of the One Way ANOVA test showed a p value = 0.931 (p>0.05), indicating no significant difference between the modified media and Sabouraud Dextrose Agar (SDA). Media with a concentration of 2% showed a colony diameter of 97.8% of the control, proving the ability of mung bean nutrition, glucose, and chitin to support fungal growth. The color of the media varied from light to dark yellow without affecting the morphology of the colony. The best growth occurred on the 4th to 14th day with a cotton-like colony texture. These results confirm the potential of modified mung bean media as an economical, environmentally friendly, and effective alternative to SDA, especially for laboratories with limited resources, as well as opening opportunities for the development of local media based on other natural materials.
Co-Authors Ahmad Muhlisin Aima Insana Ashim Manta Gayatri Gogoi Nitumani Khaklari Ratih Dewi Dwiyanti Siti Mas'odah Siti Rachmah