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All Journal Walisongo Journal of Chemistry Jurnal Dunia Farmasi
Farah Adilla
Universitas Udayana
Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

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Review: Metode Analisis Senyawa Asam Benzoat dalam Produk Makanan dan Minuman Farah Adilla
Jurnal Dunia Farmasi Vol 5, No 2 (2021): Edisi April
Publisher : Program Studi Farmasi, Fakultas Farmasi dan Kesehatan, Institut Kesehatan Helvetia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33085/jdf.v5i2.4834

Abstract

Pengawet pangan menjadi hal yang penting seiring dengan meningkatnya sifat konsumerisme masyarakat. Salah satu contoh pengawet pangan adalah asam benzoat. Konsumsi asam benzoat dalam jumlah berlebih berbahaya bagi kesehatan. Berbagai metode analisis dengan spesifisitas dan selektivitas tinggi sangat dibutuhkan untuk pengujian kandungan asam benzoat dalam produk makanan dan minuman. Prosedur analisis perlu diperhatikan agar mampu menetapkan jumlah asam benzoat yang terdapat dalam produk pangan. Studi ini menyajikan tinjauan komprehensif tentang berbagai teknik analisis yang digunakan untuk analisis pengawet asam benzoat dalam produk pangan. Metode yang digunakan yaitu studi literatur terhadap jurnal nasional dan internasional yang membahas tentang metode analisis asam benzoat dalam produk pangan. Beragam prosedur analisis memiliki kelebihan dan kekurangan berdasarkan kondisi sampel yang akan dianalisis. Berbagai metode preparasi sampel yang telah dilakukan meliputi ekstraksi cair – cair, filtrasi membran, dan ultrasonik. Metode analisis asam benzoat yang telah digunakan yaitu spektrofotometri UV-Vis, Kromatografi Gas, dan Kromatografi Cair Kinerja Tinggi. Metode analisis RP-HPLC dengan detektor UV-Vis merupakan metode yang paling banyak digunakan dalam analisis asam benzoat.
Chromatography Profiles of Terpenoid Compounds in The Extract of Sambiloto (Andrographis paniculata) Herb from Various Solvents Mohammad Fajar Hadi Salim; I Made Arie Dharma Putra Nugraha; Farah Adilla; Luh Pratiwi Diva Yanti
Walisongo Journal of Chemistry Vol 4, No 2 (2021): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v4i2.7783

Abstract

Sambiloto (Andrographis paniculata) is widely used as a medicinal plant for Indonesians. The content of extracted bioactive compounds is affected by the polarity of the extraction solvent. This study aims to determine the effect of extraction solvent on the chromatographic profile of Sambiloto herb, especially terpenoid group using the TLC-Spectrophotodensitometry method. Sambiloto herb was extracted by sonication method using methanol, ethanol, ethyl acetate, and chloroform as the solvents. Chromatographic profiles were identified by stationary phase of TLC silica gel GF254 plate and a mobile phase of chloroform:methanol (10:1 v/v), observed visually, and scanned at 210 nm. The plate was sprayed with anisadehyd-sulfuric acid reagent and observed visually. The compounds that belong to terpenoid compounds, namely S2, S5, S8, S11, and S12, had a greater percentage of peak area than the total area so that they were categorized as major components. The extraction solvent for Sambiloto herb that provides the best chromatographic profiles was found in the solvent of methanol extraction.
Co-Authors I Made Arie Dharma Putra Nugraha Luh Pratiwi Diva Yanti Mohammad Fajar Hadi Salim