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Rinto .

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Author-ID : 2512912

Agriculture, Biological Sciences & Forestry

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ANATOMI, KOMPONEN BIOAKTIF DAN AKTIVITAS ANTIOKSIDAN DAUN MANGROVE API-API (Avicennia Marina) Agoes Mardiono Jacoeb; Sri Purwaningsih; Rinto .
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14 No 2 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

The study about chemical and physical characteristics, bioactive compounds and antioxidant activity of mangrove api-api (Avicennia marina) leaf have been done. The structure of leaf tissues was analyzed with microscopic method, chemical compositions were determined by proximate analysis, antioxidant activity was determined by DPPH method, while bioactive compounds were determined by phytochemical assay. The microscopic analysis shown that api-api’s leaf have common feature dicotyl leaves structure, but it has a unique additional structure, that was salt extruding gland. The result of proximate analysis showed that api-api’s leaf was high in crude fiber (4.12%) and carbohydrate (23.00%). Ethyl acetate extract of api-api’s leaf showed higher antioxidant activity than hexane and methanol extracts, with IC value is 182.33 ppm. The ethyl acetate extract in concentration 300 ppm had higher ability to inhibite autooxidation that occurred in oil/water emulsion. Flavonoid, steroid dan reduction sugar were detected in api-api leaf extract.Keywords: antioxidant activity, Avicennia marina, DPPH, flavonoid, salt extruding gland.

KARAKTERISASI PROTEASE DARI ISOLAT BAKTERI ASAL TUMBUHAN RAWA DARI INDRALAYA Ace Baehaki; Rinto .
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 15 No 1 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Proteases constitute one of the most important groups of industrial enzymes, therefore screening of these proteases from bacteria is important. The aim of this study was to characterize proteases from plant swamp bacteria. The result of proteolytic assays revealed three isolates possesed proteolytic index >1.00 (T1P4, H1P4 and K1P4). The optimum fermentation time of each isolate was 48 hours. The optimum pH of proteases from T1P4, H1P4 and K1P4 was 8.0; 7.5; and 8.0, respectively. The optimum temperature of T1P4, H1P4 and K1P4 protease was 50 °C; 40 °C; and 50 °C, respectively. Metal ions (Fe, K, Mn, and Zn) inhibited T1P4 protease except Fe2+ (5 mM), H1P4 protease except Zn2+ (5 mM), K1P4 protease except Fe (1 and 5 mM). Effect of inhibtor specific (EDTA) shown EDTA inhibit all proteases. Study on the effect of metals ion and spesific inhibitors indicated that all protease were metaloprotease. The moleculer weights of T1P4, H1P4 and K1P4 proteases were determinited by using SDS-PAGE and zymography technique were 151; 138; and 127 kD, respectively.Key words: bacteria, characterization, protease, swamp

AKTIVITAS PENGHAMBATAN ISOLAT BAKTERI ASAM LAKTAT IKAN NILA DAN TONGKOL TERHADAP BAKTERI MERUGIKAN PRODUK PERIKANAN Rinto .; Ade Dwi Sasanti; Kusumawati Fitria
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 15 No 2 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Lactic acid bacteria were known has a potential for biopreservative and maintaining the safety of food products. The purpose of this research was to obtain lactic acid bacteria that capable to inhibit harmful bacteria in fisheries products. The stages of the methods were isolation of lactic acid bacteria from the digestive tract tilapia and frigate tuna with MRS agar + CaCO3, antibacterial test against Bacillus subtilis, Morganella morganii, and Escherichia coli using agar well diffusion method and test the alleged strengthening lactic acid bacteria. The results showed that there were two isolates of lactic acid bacteria that have the greatest inhibitory for harmful bacteria tested.Key words: antibacterial, biopreservative, inhibitor harmful bacteria, lactic acid bacteria

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