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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis
Eka Deskawati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Library & Information Science

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Karakteristik dan Aktivitas Antioksidan Rumput Laut Gracilaria sp. Asal Banten: Characteristics and Antioxidant Activities of Gracilaria sp. Seaweed from Banten Sri Purwaningsih; Eka Deskawati
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 3 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v23i3.32808

Abstract

Rumput laut Gracilaria sp. sering dimanfaatkan sebagai sumber agar-agar komersial. Penelitian ini bertujuan untuk menentukan karakteristik dari Gracilaria sp. sebagai bahan baku pangan fungsional. Metode ekstraksi pada penelitian ini yaitu maserasi tunggal menggunakan berbagai pelarut seperti: etanol, etil asetat, dan n-heksan, dengan perbandingan Gracilaria sp. dan pelarut yaitu 1:6. Parameter analisis terdiri atas proksimat, logam berat, rendemen, fitokimia, penentuan total fenol dan total flavonoid menggunakan metode spektrofotometer UV-Vis, serta aktivitas antioksidan menggunakan metode DPPH. Hasil penelitian menunjukkan kadar air 83,28% (bb), protein 9,36%, lemak 0,60%, abu 24,83% dan karbohidrat 11,05% (bk). Kadar logam berat Hg, Pb, Cd di bawah ambang batas dan tidak ditemukan logam berat As sesuai dengan SNI 2690:2015. Pengujian komponen bioaktif ekstrak etanol Gracilaria sp. secara kualitatif didapatkan alkaloid, fenol, saponin, flavonoid, triterpenoid. Kadar total flavonoid dan total fenol berturut-turut sebesar 21,78±0,32 mg QE/g dan 124±2,13 mg GAE/g. Perbedaan pelarut dalam ekstraksi berpengaruh nyata (α=0,05) terhadap aktivitas antioksidan, dengan aktivitas tertinggi adalah ekstrak etanol dengan nilai IC50 sebesar 22,15±1,63 μg/mL (sangat kuat). Hasil penelitian ini membuktikan bahwa Gracilaria sp. aman dan dapat digunakan sebagai bahan baku pangan fungsional.
CHARACTERIZATION AND TOXICITY TEST OF PUFFER FISH FROM PAMEUNGPEUK WATERS, WEST JAVA Eka Deskawati; Sri Purwaningsih; Purwanti Ningsih
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 6 No. 1 (2014): Electronik Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (213.924 KB) | DOI: 10.29244/jitkt.v6i1.8631

Abstract

A puffer fish contains a secondary metabolite toxic called tetrodotoxin (TTX), which can be used as an anesthetic and medication to relieve chronic pain for cancer patients. The purposes of this study were to determine the LC50 value and chemical composition of extracts from various tissues of puffer fishes as a first step to determine the further isolation of tetrodotoxin. In this study various tissues from meat, liver, skin, heart, and ovary of Arothron hispidus and Diodon hystrix species were extracted, characterized, and tested levels of toxicity using Brine Shrimp Lethality Test (BSLT) methods. Between the two puffer fishes studied, the highest toxicity was found in Arothron hispidus ovary with LC50 value of 29.65 ppm. Chemical constituents of the ovary were alkaloids, carbohydrates, and amino acids. Toxic compounds found in the extracts of puffer fish ovaries were tetrodotoxin which is a compound of the alkaloid class of secondary metabolites. Keywords: Puffer fish, Arothron hispidus, Diodon hystrix, toxicity, Brine Shrimp Lethality Test
Co-Authors Purwanti Ningsih Sri Purwaningsih