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Raden Wasito
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Biochemistry, Genetics & Molecular Biology Veterinary

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Journal : Jurnal Veteriner

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Diet Fosfor Tinggi Penyebab Osteodistrofia Fibrosa pada Tikus (HIGH PHOSPHOROUS DIET CAUSED OSTEODISTROFIA FIBROSA IN RATS) Hartiningsih .; Raden Wasito
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this research was to study the impact of high phosphor diet on the femur of ratsconsuming soybean. Ten female rats at 4 weeks of age were randomly divided into two groups of five, NP(rats fed with normal phosphor diet) and HP (rat fed with high phosphor diet). Each rats was placed intoindividual cages at 22-25°C. All  rats were given normal diet and water which were  provided ad libitum.The rats were also adaptation for three weeks before the treatment was given.  At seven weeks of age, ratsin NP group were fed normal diet (calcium:phosphor=0,6%:0,4%), while rats in HP group were fed highphosphorus diet (calcium:phosphor=0,6%:3,6%) for 6 weeks. At the end of the study, blood was collectedfrom plexus retroorbitalis for calcium and phosphor analysis, while right femur was taken forhistopathological examination using hematoxylin and eosin stain. The research results showed that bloodof calcium was significantly reduced (P<0.01) in HP group compared with NP group, while blood phosphorwas significantly increased (P<0.01) in HP group. Histopathological analysis of the proximalis femur inNP group showed the osteogenic zone of physis and the trabecular bone speculum of metaphysis werenormal, while the osteogenic zone of physis and the trabecular bone speculum of metaphysis in HP groupwere irregular.  Fibroblast in  trabecular bone speculum of bone marrow were also observed.  It can beconcluded that high phosphorus diet may cause osteodystrophia fibrosa in rats.
Pelacakan Virus Bovine Viral Diarrhea pada Darah yang Dikoleksi dengan Kertas Saring Flinders Technology AssociatesTM (DETECTION OF BOVINE VIRAL DIARRHEA VIRUS FROM BLOOD SAMPLES COLLECTED USING FLINDERS TECHNOLOGY ASSOCIATESTM CARDS) Hastari Wuryastuti; Raden Wasito; Prabowo Purwono Putro
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bovine viral diarrhea virus (BVDV) is a common pathogen that causes major economic losses to dairyindustry worldwide. Amplification of 5’-UTR regions of BVDV genome from blood samples usingreversetranscriptase polymerase chain reaction (RT-PCR) is one of sensitive and accurate techniques forBVDV detection. A common limitation for molecular detection of virus is the ability to obtain high qualityof nucleic acid in the samples. Inappropriate storage conditions will result in a false negative test. FiveEDTA-blood samples collected from BVDV persistently infected cattle were used in the present study.Ten repetitions were done for each sample. The blood were then dripped in the middle part of the FlindersTechnology AssociatesTM (FTA) cards and let it dried for approximately 2 hours. The effectiveness of theFTATMcards for storage and retrieval of BVDV RNA from blood samples molecularly using RT-PCRtechnique were tested and evaluated. The results proved that FTATM cards are effective and safe for thestorage of BVDV genome for a long period at room temperature. Based on the result of the present study,it can be concluded that in veterinary medicine field, the application of FTATM technology needs to bedeveloped to various type of samples and should be used routinely for collecting samples containinginfectious agents.
Co-Authors Arif Setiani Wahyuning Tyas Hartiningsih . Hastari Wuryastuti Hastary Wuryastity Prabowo Purwono Putro