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AQ-8 Detection of the Foot and Mouth Disease in Buffalo Meat Originating From India with the Reverse-Transcription PCR Method Hestu Prastanti; . Marjono; Melyna Sandra; . Kasidi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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The foot and mouth disease (FMD) is still a major issue in the world of animal health. To date, not many countries are free from FMD; Indonesia is a country that has been FMD-free since 1986 and has been acknowledged by the World Animal Health Organization (OIE) from 1990.  This success was not easily obtained, as a huge amount of energy, funds, and thoughts were expended over a period of nearly one hundred years. The regional situation in Asia at present is still a huge threat to the potential of FMD entry. Malaysia, Thailand, and India are risks for the re-entry of the disease to Indonesia.There are constant efforts to smuggle meat in from regional Asian countries. The eastern coast of Sumatra is still a favorite area for importing meat illegally from countries not yet FMD-free. Moreover, the import of frozen boneless meat from FMD-free zones in India is still a threat and could potentially spread FMD to our country. Therefore, there need to be anticipation efforts and an early detection ability to identify the FMD virus which might be carried by imported meat. The meat that enters illegally and comes from FMD-free zones in India has the potential for bringing and spreading FMD; The lack of a specific method to be used as a tool for detection of FMD through imported or illegal meat.The purpose of this method development activity is to acquire a suitable, quick, and highly accurate method in detection of FMD which might be carried by imported or illegal meat; one of these methods is assessment using Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). This method is expected to become a standard method in FMD testing in the Agricultural Quarantine Agency.
AQ-12 Application of a Multiplex PCR Assay to Detect Campylobacter fetus subspecies venerealis from Imported Bovine Preputial Samples Mazdani Daulay; Adi Komara; Novera Nirmalasanti; Siti Khadijah; . Marjono; Melyna Sandra; Muhamad Taopik; . Mukromin; . Mustamil; Rahmat Setya Adji
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Campylobacteriosis, caused by Campylobacter spp., is of considerable economic importance to the cattle industry worldwide. Campylobacter spp. were recognized as etiological agents of abortion in sheep. Campylobacter fetus subsp. fetus (Cff) causes sporadic abortion in sheep, often late in gestation, while subspecies venerealis (Cfv) is a cause of sexually transmitted bovine infertility and sporadic abortion in cattle. Various investigations have been carried out in different countries to assess the prevalence and impact of this disease. Some published results surveys are outlined in Table 1. Table 1. A summary of published data showing the prevalence of C. fetus in different countriesStudyareaSample type(s)Sample sizePrevale nce ofC. fetus (%)Diagnostic methodAustralia(1985-1986) Bulls (preputi al suction)1 008animals41 herds87% herdspositiveSerological(Fluorescentantibody test) California(United States of America)Cows40047Serological(ELISA) New ZealandCows (vaginal mucous) and bulls (preputial wash)1 230 cows(125 herds)54 bulls70% herds positiveCfv : 0Cff/othersSerological(ELISA)Bacteriological culture          Cff: Campylobacter fetus subsp. fetus, Cfv: C. fetus subsp. venerealis. According to [1], Bovine Genital Campylobacteriosis (BGC) disease was classified as 1st Group of animal quarantine disease. It is an exotic disease that was not ever detected in Indonesia. However, large scale cattle importation to Indonesia from the countries which ever reported BGC prevalence in their territories, initiating and spreading BGC will be the major threat for feedlotter or dairy farm in Indonesia. Hence, we should apply diagnostic test to detect Cfv in order to prevent the introducing the BGC to Indonesia. The aim of this study was to verify that multiplex PCR assay applicative to detect Campylobacter fetus subsp. venerealis from field samples.