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All Journal Journal of Dentistry Indonesia Padjadjaran Journal of Dental Researchers and Students
Anggraini Margono
Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Medicine & Pharmacology Public Health

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Time and Dose Dependent Effects of Adipose-Derived Stromal Vascular Fraction Exosomes on Human Dental Pulp Stem Cell Proliferation Alinda, Sylva Dinie; Margono, Anggraini; Julianto, Indah; Bagio, Dini Asrianti
Journal of Dentistry Indonesia Vol. 33, No. 1
Publisher : UI Scholars Hub

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Abstract

Background: Cell proliferation is essential for tissue repair and immunomodulation in regenerative dentistry. Adipose-derived stromal vascular fraction (AD-SVF) is a promising autologous source for tissue engineering. Its exosomes (AD-SVF Exo), as cell-free products stable under ischemic conditions, represent an ethical and attractive modality for dental tissue regeneration and broader regenerative applications. Prior study indicates that AD-SVF Exo can enhance human dental pulp stem cell (hDPSC) migration and proliferation, particularly at low concentrations, but their detailed time- and dose-dependent proliferative effects remain unclear. This study aimed to investigate the time- and dose-dependent effects of low-concentration AD-SVF Exo on hDPSC proliferation in vitro. Methods: AD-SVF exosomes were isolated by size-exclusion chromatography and characterized by flow cytometry and nanoparticle tracking analysis, then applied to hDPSCs at multiple concentrations. Proliferation was evaluated using the Cell Counting Kit-8 assay. One-way ANOVA with LSD post hoc testing was performed (α = 0.05). Results: AD-SVF exosomes (mean size 103 ± 24 nm) expressed CD9 and CD63. All tested concentrations significantly increased hDPSC proliferation versus control in a time- and dose-dependent pattern, with lower doses generally producing the most favorable responses. Conclusions: AD-SVF exosomes enhance hDPSC proliferation in a time- and dose-dependent manner, supporting their potential as a cell-free adjunct in regenerative dentistry.
Hubungan Antara Pengetahuan dan Sikap Mahasiswa tentang Pencegahan COVID-19 dengan Praktik Pelayanan di RSKGM FKG UI Studi pada Mahasiswa Program Pendidikan Dokter Gigi Spesialis Konservasi Gigi Ricardo, Shalina; Amalia, Adisvia Ramadhanty; Margono, Anggraini
Padjadjaran Journal of Dental Researchers and Students Vol 6, No 1 (2022): Februari 2022
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v6i1.36179

Abstract

Latar Belakang: Pandemi COVID-19 di Indonesia memiliki dampak besar pada praktik pelayanan kedokteran gigi. Dokter gigi memiliki risiko lebih tinggi terinfeksi COVID-19 dalam prosedur kedokteran gigi. Oleh karena itu, perlu dilakukan tindakan pencegahan penularan COVID-19 dalam bentuk kontrol infeksi yang merupakan salah satu indikator penilaian kualitas dalam praktik pelayanan kedokteran gigi. Tujuan: Penelitian ini menjelaskan hubungan antara pengetahuan dan sikap tentang pencegahan COVID-19 dengan praktik pelayanan di RSKGM FKG UI pada mahasiswa PPDGS Konservasi Gigi. Metode: Penelitian deskriptif analitik potong-lintang pada 58 mahasiswa PPDGS Konservasi Gigi FKG UI berbasis kuesioner yang dibagikan secara daring. Hasil: Berdasarkan Uji Mann-Whitney dan Kruskal Wallis, tidak terdapat perbedaan bermakna secara statistik (p > 0,05) antara pengetahuan, sikap dan praktik pencegahan COVID-19 berdasarkan lama pengalaman kerja dan tahun angkatan pendidikan. Uji korelasi Spearman menunjukkan tidak ada hubungan (p > 0,05) pengetahuan dan sikap mahasiswa tentang pencegahan COVID-19 terhadap praktik pelayanan kedokteran gigi. Kesimpulan: Mahasiswa PPDGS Konservasi Gigi memiliki tingkat pengetahuan baik, sikap positif dan praktik yang baik terkait pencegahan COVID-19. Tidak ada hubungan antara pengetahuan dan sikap responden tentang pencegahan COVID-19 dengan praktik pelayanan di RSKGM FKG UI serta tidak terdapat perbedaan yang bermakna pengetahuan, sikap, dan praktik pencegahan COVID-19 berdasarkan lama pengalaman kerja dan tahun angkatan pendidikan.
Co-Authors Alinda, Sylva Dinie Amalia, Adisvia Ramadhanty Bagio, Dini Asrianti Indah Julianto, Indah Ricardo, Shalina