Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Serambi Biologi
Deratih Bunga Purwakasih
Padang State University
Agriculture, Biological Sciences & Forestry Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Primer Design and in Silico PCR for Detection Shigella Sp. on Refilled Water Samples Deratih Bunga Purwakasih
Serambi Biologi Vol 6, No 1 (2021): Serambi Biologi
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/11072RF00

Abstract

Refillable drinking water is an effort to meet the needs of drinking water for consumption by modern society. One of the quality parameters of drinking water suitable for consumption is not contaminated with Escherichia coli, Salmonella sp. and Shigella sp. Conventional microbiological tests were often conducted for the detection of water pathogenic bacteria, including culturing the bacteria in medium culture and biochemical assay, but this method requires a long working time and expensive costs. Currently, Polymerase Chain Reactions (PCR) can be an alternative method because it has high accuracy. Primers are one of the important components of PCR so they must be specifically designed to ensure the success of DNA amplification. This study aimed to obtain the specific sequence of PCR primer for detection Shigella-contaminated refillable drinking water sample and conducted the in silico PCR. Primers were designed in silico using Primer BLAST in NCBI (National Center for Biotechnology Information) and Geneious Prime for in silico PCR. To get a Shigella-specific primer, pairwise alignment of Shigella sp. (NC_004337.2) and E.coli (NC_000913.3) was performed and specific sequence of Shigella was used as primer candidates. The result of this study were the Shigella-specific sequence PCR primer forward 5'-GCTAATGAAAATGGCGCTGT-3' and reverse 5'-AGCCGACGGTTTGAAGTTAC-3' with PCR product length of 815 bp.
Co-Authors