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All Journal INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Green Medical Journal
Andina Putri Aulia
Department of Clinical Pathology, Faculty of Medicine, Universitas Islam Sultan Agung, Semarang, Indonesia
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences

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A POWERFUL ELISA TECHNIQUE TO TEST THE POTENTIAL OF EXTRA VIRGIN OLIVE OIL IN REDUCING TNF-Α LEVEL AND EDEMA VOLUME IN MALE Rattus norvegicus EXPOSED TO CARRAGEENAN Andina Putri Aulia; Suprapto Maat; Aryati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i1.1400

Abstract

Extra virgin Olive oil is extracted from fruit that can be used as anti-inflammatory agent. This research aimed to test the potential of extra virgin olive oil in reducing edema volume and TNF-α plasma in carrageenan-induced rats. This research was purely experimental research with the post test control group design. A total of twenty eight Wistar rats were divided randomly into four treatment groups. Group I was a control negative group, while the group II, III, and IV were orally administered with extra virgin Olive oil at the dose of 0.9 ; 1.8 ; 2.7 mL/day, respectively. Paw edema was measured one hour before the rats was induced to carrageenan and every hour until four hours after it was induced to carrageenan. TNF-α plasma was measured at four hours. Analysis of the data was done by calculating the presentation of edema inhibition in every group, then the data was statistically analyzed by Anova, Repeated Anova, LSD and Kruskal Wallis test with 95% confidence interval. The result showed that extra virgin olive oil has an anti-inflammatory effect. The highest decrease in edema volume percentage was in group III (14.21%). There was a significant difference in the edema volume of all treatment groups at each time of the experiment with TNF-α value (p < 0.05). In conclusion, the administration of extra virgin olive oil can lower the volume of carrageenan-induced edema in rats depend on the dose. Also, the administration of extra virgin olive oil can be dose-dependent in reducing the levels of TNF-α in carrageenan-induced edema in rats.
Serum Uric Acid and Glycemic Control among Diabetes Mellitus Patients in Semarang, Indonesia: A Hospital-Based Cross-Sectional Study Rahmadani Ayu Azari; Hesty Wahyuningsih; Andina Putri Aulia; Minidian Fasitasari; Ajat Sudrajat; Bagas Priambodo
Green Medical Journal Vol 8 No 2 (2026): Green Medical Journal
Publisher : Fakultas Kedokteran Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/7hs7je26

Abstract

Introduction: The relationship between serum uric acid (SUA) and glycemic status in established diabetes is biologically plausible but inconsistent across studies because insulin resistance, renal urate handling, glycosuria, obesity, kidney function, and glucose-lowering therapy can influence SUA in different directions. This study evaluated the association of SUA with fasting blood glucose (FBG) and glycated hemoglobin (HbA1c) in a hospital-based sample in Semarang, Indonesia. Methods: This retrospective cross-sectional study screened 410 medical records from Sultan Agung Islamic Hospital, Semarang, during 2018–2020. Thirty records with complete SUA, FBG, and HbA1c data were available for analysis. Hyperuricemia was defined as SUA >7.0 mg/dL in men and >6.0 mg/dL in women. The revision reanalyzed the available raw dataset using Shapiro–Wilk testing, Spearman correlation, exploratory partial Spearman correlation adjusted for age, sex, and recorded obesity status, and Fisher’s exact test. Two-sided p<0.05 was considered statistically significant. Results: The mean age was 54.3±6.5 years; 16/30 (53.3%) were women and 18/30 (60.0%) met the sex-specific definition of hyperuricemia. SUA showed a moderate positive monotonic association with FBG (Spearman ρ=0.401, 95% bootstrap CI 0.053 to 0.665, p=0.028); this remained similar after adjustment for age, sex, and obesity (partial ρ=0.396, p=0.041). The association with HbA1c was weak and not statistically significant (ρ=0.270, 95% bootstrap CI -0.126 to 0.624, p=0.149). Conclusion: Higher SUA was associated with higher FBG, but not HbA1c. These exploratory findings should be interpreted cautiously because of the small, highly selected sample and incomplete data on renal function, medication use, diabetes duration, and other confounders.
Co-Authors Ajat Sudrajat Aryati Aryati Bagas Priambodo Hesty Wahyuningsih Minidian Fasitasari Rahmadani Ayu Azari Suprapto Maat