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Journal : Journal of Applied Chemical Science

The Moringa Seed (Moringa oleifera, L.) Extracts Potentially Reduce the Effects of Clothes Washing Wastewater on Growth of Green Beans (Vigna radiata) Merymistika Y. Afred; Bibiana D. Tawa; Pius D. Ola
Journal of Applied Chemical Science Volume 1, No. 2 , 2012
Publisher : Journal of Applied Chemical Science

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Abstract

A study concerning the use of Moringa seed (Moringa oleifera, L.) extracts to reduce the influence of clothes washing wastewater on growth of green bean (Vigna Radiata) has been conducted. The study aimed to determine the effective dose of coagulants prepared from moringa seed extracts (400, 600, 800, 1000, and 2000 mg/L) on germination of green beans in wastewater. The results showed that the optimum dose of coagulant was 2000 mg/L. The treatments with various doses of coagulants indicated significantly different except for the dose of 1000 mg/L and 2000 mg/L (P < 0.05). The stem height and leaf watering with coagulant water were 25.9 cm and 14.1 cm2, respectively, while by the tap water were 24.3 cm and 10.99 cm2, respectively. Those results conclusively indicated that the water coagulant prepared from moringa seed extracts promotes growth of the vigna radiata.
Antioxidant Activities of Chloroform and Aqueous Fractions of Myrmecodia Pendenss Extract: A Preliminary Study Yohanes Buang; Ermelinda Noya; Pius D. Ola; Theo Da Cunha
Journal of Applied Chemical Science Volume 2, No. 1 , 2013
Publisher : Journal of Applied Chemical Science

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Abstract

There have been elucidated the antioxidant activities of chloroform and aqueous fractions of methanol extracts isolated from myrmecodia pendenss. The study was firstly started by maceration of myrmecodia pendenss powder in certain quantity of pure methanol solvent and followed by gradual partition of the methanol extract with chloroform and distillated water and then purified with column chromatography. The chemical contents migrated into both those later solvents were detected by GC-MS and their functional groups were detected by FT-IR instruments. Thereafter, the antioxidant activities of the migrated compounds in each those solvents were determined using hydrogen peroxide protocol and the absorbance was measured at 240 nm. The ascorbic acid solution was used as control. The results showed that FT-IR spectrum showed methylene and ethene groups are existed within the compounds contained by the extracts as well as the hydroxyl, carbonyl, aromatic, and keton groups. Furthermore, the GC-MS spectrum indicated the compounds that are existed within the chloroform and the aqueous fractions of the extract. The average absorbances of compounds contained within the chloroform and aqueous fractions were 0.161 and 0.112, respectively, whereas that of the control was 0.085. Those absorbance data conclusively indicated that higher absorbance higher residue of chromophore content existed within the fraction and higher failures of hydroxylation reactions. It was however, the antioxidant activities of the aqueous fractions were higher than those of the chloroform fractions.