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Yohanes Buang
Department of Chemistry Faculty of Science and Engineering, Nusa Cendana University
Chemical Engineering, Chemistry & Bioengineering Chemistry

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The effects of conjugated linoleic acid on growth and lipid profiles in serum and liver tissue of type-2 diabetes mellitus model rat Yohanes Buang; Yu-Ming Wang; Teruyoshi Yanagita
Journal of Applied Chemical Science Volume 1, No. 1 , 2012
Publisher : Journal of Applied Chemical Science

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Conjugated linoleic acid (CLA) refers to a group of positional and geometric conjugated-dienoic isomers of linoleic acid. This chemical has been suggested to have antidiabetic activity. However, the effects of CLA on growth parameters and lipid profiles in serum and liver tissue of type-2 diabetic subjects have no data reported. The present study was conducted to elucidate the effects of CLA on growth parameters and the lipid profiles in serum and liver tissue of male Otsuka Long-Evans Tokushima Fatty rat, as a model of type-2 diabetes. The final body weight and the liver weight were nearly similar between the groups but the white adipose tissue (WAT) weight decreased, although it failed to reach significant level (P<0.05). Serum high density lipoprotein levels moderately increased, it was approximately by 6%, in which the increase was accompanied with a reduction of atherogenic index slightly (Control = 1.00±0.02, while the treatment group = 0.95±0.03). Furthermore, the liver triglycerides (TG) content decreased by 13%. The similar final body weight accompanied with reductions of WAT level and liver TG content might indicate that CLAs diets have management properties on body fat and liver TG metabolism in patients with type-2 diabetes mellitus.
The Antioxidant Compounds Contained in n-Butanol and Aquamor Fractions of Dischidia Nummumaria Extract Abner A. Leba; Sherly M. F. Ledoh; Yohanes Buang
Journal of Applied Chemical Science Volume 1, No. 1 , 2012
Publisher : Journal of Applied Chemical Science

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There have been elucidated the antioxidant compounds contained in n-butanol and aquamor fractions of methanol extracts isolated from dischidia nummularia. The study was firstly started by maceration of dischidia nummularia powder in certain quantity of pure methanol solvent and followed by gradual partition with n-butanol and aquamor water and then purified with column chromatography. The chemical contents migrated into both those later solvents were detected by LC-MS and their functional groups were detected by FT-IR instruments. The results showed that methanol extract and those n-butanol and aquamor fractions contained 24, 14, and 15 compounds, respectively. The compounds contained in those three samples involve myricetin, quercetin, kaempferol, quercetin-3-ogalactoside, quercetin-3-orhamnoside, quercetin-3-oglucoside, kaemp-ferol galactoside, kaempferol-3- oglucoside, luteolin, and catechin. These compounds have been reported to have behaviors as antioxidant as reviewed in various literatures (see result and discusion). Furthermore, the FT-IR spectrum provides information regarding the functional group and element group of those antioxidant compounds. Based on those reports therefore the methanol extract and the n-butanol and aquamor fractions of dischidia nummularia isolated have those antioxidant compounds.
Antioxidant Activities of Chloroform and Aqueous Fractions of Myrmecodia Pendenss Extract: A Preliminary Study Yohanes Buang; Ermelinda Noya; Pius D. Ola; Theo Da Cunha
Journal of Applied Chemical Science Volume 2, No. 1 , 2013
Publisher : Journal of Applied Chemical Science

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There have been elucidated the antioxidant activities of chloroform and aqueous fractions of methanol extracts isolated from myrmecodia pendenss. The study was firstly started by maceration of myrmecodia pendenss powder in certain quantity of pure methanol solvent and followed by gradual partition of the methanol extract with chloroform and distillated water and then purified with column chromatography. The chemical contents migrated into both those later solvents were detected by GC-MS and their functional groups were detected by FT-IR instruments. Thereafter, the antioxidant activities of the migrated compounds in each those solvents were determined using hydrogen peroxide protocol and the absorbance was measured at 240 nm. The ascorbic acid solution was used as control. The results showed that FT-IR spectrum showed methylene and ethene groups are existed within the compounds contained by the extracts as well as the hydroxyl, carbonyl, aromatic, and keton groups. Furthermore, the GC-MS spectrum indicated the compounds that are existed within the chloroform and the aqueous fractions of the extract. The average absorbances of compounds contained within the chloroform and aqueous fractions were 0.161 and 0.112, respectively, whereas that of the control was 0.085. Those absorbance data conclusively indicated that higher absorbance higher residue of chromophore content existed within the fraction and higher failures of hydroxylation reactions. It was however, the antioxidant activities of the aqueous fractions were higher than those of the chloroform fractions.
Antibacterial Activities of Ranting Patah Tulang (Euphorbia tirucalli) Extract: Short Communication Sesilia I. Napa; Theo Da Cunha; Yohanes Buang
Journal of Applied Chemical Science Volume 2, No. 1 , 2013
Publisher : Journal of Applied Chemical Science

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There have been studied the antibacterial activities of ranting patah tulang (euphorbia tirucalli) extracts against the growth of salmonella thyposa (gram negative) bacteria. The study was firstly started to isolate the antibacterial components of euphorbia tirucalli that was soluble in methanol. Thereafter, the methanol solvent was evaporated to yield powder and the powder was used to prepare the extract concentrations in each petri dish as of following levels that were 10.0, 15.0, 20.0, 25.0, 50.0, 80.0, and 100.0 %, respectively. The 0.0 % of euphorbia tirucalli extracts and 1.0 % of erythromycin in each petri dish served as the controls. The results showed that the clear zone of petri dish containing the bacteria and each those extracts were 36.7±5.8, 40.0±10.0, 43.3±5.8, 60.0±10.0, 66.7±5.0, 70.0±10.0, and 76.7±5.8 mm, respectively. The clear zone of both those controls containing the bacteria were 63.3±5.8 mm. Those results indicate that higher extract concentration higher the clear zone area of the petri dish. Therefore, the euphorbia tirucalli extract potentially inhibits the growth of the salmonella thyposa (gram negative) bacteria.
Co-Authors Abner A. Leba Ermelinda Noya Pius Dore Ola Sesilia I. Napa Sherly M. F. Ledoh Teruyoshi Yanagita Theo Da Cunha Yu-Ming Wang