Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Biodjati
Rosita Fitrah Dewi
Program Studi Tadris Biologi, Institut Agama Islam Negeri Jember
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Purification and Characterization of Cellulase of Mold Isolated from Vermicomposting Process of Palm Oil Empty Fruit Bunches Rosita Fitrah Dewi; Kahar Muzakhar
Jurnal Biodjati Vol 3, No 1 (2018): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v3i1.2292

Abstract

In previous investigation of vermicomposting process of palm oil empty fruit bunches, five cellulase-producing microorganisms had been isolated. An isolated VTM1 identified as Aspergillus sp. as a cellulase producing mold, and proven produced extracellular cellulase in solid state fermentation of palm oil empty fruit bunches. The obtaining crude cellulase was purified to homogeneity by ammonium sulfate precipitation, gel filtration on Sephadex G-100 chromatography, and finally purified on ion exchange chromatography on DEAE Cellulofine A-500. The yield and purification fold were 12.89% and 107.50, respectively. The purified cellulase was easily released glucose when 5% CMC applied as substrate, as shown by TLC analysis. The purified cellulase had an optimal pH and temperature at 4.0 and 45°C, and was stable at pH 3-7 and 30-50°C, respectively.
Co-Authors Kahar Muzakhar