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All Journal Borneo Journal of Medical Laboratory Technology Lontara: Journal of Health Science and Technology Pharmaqueous Lontara Journal Of Health Science And Technology
Andri Sukeksi
Universitas Muhammadiyah Semarang
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Potensi Ekstrak Etanol Daun Sirih (Piper Betle L.) Sebagai Pengganti ADP (Adenosine Diphosphate) Pada Pemeriksaan Agregasi Trombosit Andri Sukeksi; Rizqi Yogania Rahmawati
Lontara Journal of Health Science and Technology  Vol 2 No 1 (2021): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v2i1.184

Abstract

Adenosine diphosphate (ADP) reagent is often used in the examination of platelet aggregation, yet has limitations in terms of price and availability. Natural source namely betel leaf (Piper betle L.) are believed to be used as a substitute for ADP because betel leaf have the characteristics of styptic (to resist bleeding), vulnerary (to heal skin wounds), and anti-inflammatory (to preserve inflammation) (Moeljanto & Mulyono, 2003). The aim of this study was to determine the potential of betel leaf on the examination of platelet aggregation. The method used in this research included the betel leaf extraction using ethanol as solvent with an evaporator. The extract obtained was used as a substitute for the ADP reagent in platelet examination in the form of determining the percentage of platelet aggregation in 16 human blood samples, with a population of students at the University of Muhammadiyah Semarang. The final results showed that the addition of betel leaf extraction to the 16 blood samples examined gave an average value of platelet aggregation percentage which was higher (65%) than the mean value of platelet aggregation using ADP reagent mixing (57%). Both results fall within the normal value range, namely 50-70%. It can be concluded that betel leaf extract has the potential to be used as a replacement reagent for ADP in the process of examining platelets, especially in determining the percentage value of platelet aggregation.
Potensi Ekstrak Etanol Daun Sirih (Piper Betle L.) Sebagai Pengganti ADP (Adenosine Diphosphate) Pada Pemeriksaan Agregasi Trombosit Andri Sukeksi; Rizqi Yogania Rahmawati
Lontara Journal of Health Science and Technology Vol 2 No 1 (2021): Ilmu dan Teknologi Kesehatan
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat Politeknik Kesehatan Muhammadiyah Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53861/lontarariset.v2i1.184

Abstract

Adenosine diphosphate (ADP) reagent is often used in the examination of platelet aggregation, yet has limitations in terms of price and availability. Natural source namely betel leaf (Piper betle L.) are believed to be used as a substitute for ADP because betel leaf have the characteristics of styptic (to resist bleeding), vulnerary (to heal skin wounds), and anti-inflammatory (to preserve inflammation) (Moeljanto & Mulyono, 2003). The aim of this study was to determine the potential of betel leaf on the examination of platelet aggregation. The method used in this research included the betel leaf extraction using ethanol as solvent with an evaporator. The extract obtained was used as a substitute for the ADP reagent in platelet examination in the form of determining the percentage of platelet aggregation in 16 human blood samples, with a population of students at the University of Muhammadiyah Semarang. The final results showed that the addition of betel leaf extraction to the 16 blood samples examined gave an average value of platelet aggregation percentage which was higher (65%) than the mean value of platelet aggregation using ADP reagent mixing (57%). Both results fall within the normal value range, namely 50-70%. It can be concluded that betel leaf extract has the potential to be used as a replacement reagent for ADP in the process of examining platelets, especially in determining the percentage value of platelet aggregation.
Pengaruh Penundaan Sampel Terhadap Nilai Agregasi Trombosit Darah Sitrat Pada Suhu Ruang (25ºC) Gela Setya Ayu Putri; Iha Himatul Ulya; Andri Sukeksi
Borneo Journal of Medical Laboratory Technology Vol. 8 No. 1 (2025): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v8i1.10651

Abstract

Pemeriksaan agregasi trombosit merupakan pemeriksaan penting untuk mengevaluasi fungsi trombosit. Pemeriksaan ini bersifat sangat sensitif dan dipengaruhi oleh berbagai faktor, seperti konsentrasi sodium sitrat, jumlah trombosit, suhu penyimpanan, dan waktu penundaan pemeriksaan. Metode pemeriksaan dapat dilakukan secara otomatis maupun manual, salah satunya menggunakan metode Velaskar. Permasalahan yang sering terjadi di laboratorium adalah penundaan pemeriksaan, yang berpotensi menyebabkan kerusakan fungsi trombosit sehingga meningkatkan persentase agregasi. Penelitian ini bertujuan mengetahui perbedaan persentase agregasi trombosit berdasarkan variasi waktu penundaan sampel darah sitrat pada suhu ruang (25°C). Desain penelitian yang digunakan adalah analitik eksperimental. Sampel darah vena diperoleh dari enam responden, kemudian dibagi ke dalam empat kelompok perlakuan yaitu kelompok segera diperiksa (kontrol), ditunda 2 jam, 3 jam, dan 4 jam. Agregasi trombosit diperiksa menggunakan metode Velaskar dengan pewarnaan Giemsa. Data dianalisis dengan One-Way Anova dan Post-Hoc. Hasil penelitian menunjukkan rata-rata persentase agregasi trombosit pada kelompok kontrol, penundaan 2 jam, 3 jam, dan 4 jam berturut-turut adalah 56,3%, 59,6%, 71,5%, dan 75,5%. Analisis One-Way Anova menunjukkan nilai signifikansi 0,000 (p<0,05), menandakan adanya perbedaan bermakna antar kelompok. Uji Post Hoc mengungkap bahwa tidak terdapat perbedaan signifikan antara kelompok kontrol dan penundaan 2 jam (p>0,05), sedangkan penundaan 3 dan 4 jam menunjukkan perbedaan yang signifikan (p<0,05). Dengan demikian, pemeriksaan agregasi trombosit masih dapat dilakukan hingga 2 jam setelah pengambilan sampel pada suhu ruang, namun penundaan lebih dari 2 jam tidak direkomendasikan karena dapat memengaruhi akurasi hasil.
The Differences in HbA1c Levels in EDTA Blood Samples and Garlic Filtrate Blood Samples Arista Kurniasari Budi Fristiani; Azana Lathifa Ramadhani; Andri Sukeksi
Pharmaqueous: Jurnal Ilmiah Kefarmasian Vol. 8 No. 1 (2026): Vol. 8 no. 1 Mei 2026
Publisher : Universitas Al-Irsyad Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36760/jp.v8i1.763

Abstract

Hemoglobin A1c (HbA1c) is an important parameter used to evaluate long-term glycemic control in patients with diabetes mellitus. The standard sample for HbA1c examination is whole blood with EDTA anticoagulant. However, limitations in the laboratory encourage the exploration of natural alternatives such as garlic (Allium sativum), which contains bioactive compounds with anticoagulant properties. This study aims to determine the difference in HbA1c levels between EDTA blood samples and blood samples treated with garlic filtrate. This experimental study involved 16 samples. Blood was divided into two groups: EDTA and garlic filtrate (40 µL/mL). HbA1c levels were measured using an Alere Afinion analyzer with the boronate affinity chromatography method. Data were analyzed using the Shapiro-Wilk normality test and the Paired Samples T-test. The results showed that the average HbA1c level in EDTA samples was 5.18%, while in garlic filtrate samples it was 5.74%. Statistical analysis showed a significant difference (p = 0.000 < 0.05). This indicates that although garlic filtrate has anticoagulant potential, it does affect HbA1c measurement results.
Co-Authors Arista Kurniasari Budi Fristiani Azana Lathifa Ramadhani Gela Setya Ayu Putri Iha Himatul Ulya Rizqi Yogania Rahmawati