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PENGARUH SALINITAS TERHADAP PERTUMBUHAN, BIOMASSA DAN KLOROFIL-a DUNALIELLA SP. Muhammad Fakhri; Arning Wilujeng Ekawati
JFMR (Journal of Fisheries and Marine Research) Vol 4, No 3 (2020): JFMR VOL 4 NO 3
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2020.004.03.12

Abstract

Dunaliella sp. merupakan mikroalga hijau yang kaya beta karoten dan klorofil, bersifat euryhaline, dan telah banyak dimanfaatkan untuk budidaya ikan dan udang. Salah satu strategi yang dilakukan untuk meningkatkan produktivas mikroalga adalah dengan melakukan optimasi kondisi lingkungan. Salinitas merupakan factor esensial yang mempengaruhi pertumbuhan dan kandungan biokimia Dunaliella. Tujuan penelitian ini adalah untuk menjelaskan pengaruh salinitas yang berbeda dan menentukan salinitas terbaik untuk pertumbuhan, produksi biomassa, dan klorofil-a Dunaliella sp. salinitas yang berbeda yaitu 5 ppt, 15 ppt, 25 ppt, dan 35 ppt digunakan pada penelitian ini. Hasil dari penelitian ini menunjukkan bahwa salinitas yang berbeda berpengaruh terhadap pertumbuhan, produksi biomassa, dan klorofil-a Dunaliella sp. (p<0,05). Salinitas terbaik untuk pertumbuhan, produksi biomassa dan klorofil-a Dunaliella sp. diperoleh pada salinitas 15 ppt yang menghasilkan konsentrasi sel maksimum sebesar 19,86 x 106 sel mL-1, biomassa 0,52 g l-1, dan klorofil-a 11,26 mg/l. Laju pertumbuhan yang tinggi berhubungan dengan biomassa dan kandungan klorofil-a Dunaliella sp. Dapat disimpulkan bahwa salinitas yang berbeda memberikan pengaruh signifikan terhadap pertumbuhan, produksi biomassa, dan klorofil-a Dunaliella sp. Disarankan menggunakan salinitas 15 ppt untuk menghasilkan pertumbuhan, produksi biomassa dan klorofil-a yang optimal.
Improvement Of Feed Utilization And Survival Rate Of Clarias Gariepinus Using Probiotic And Fermented Feed In Cemorokandang District Muhammad Fakhri; Arning Wilujeng Ekawati
Journal of Innovation and Applied Technology Vol 5, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2020.005.02.1

Abstract

The common problem faced by catfish culturing in Cemorokandang District is high feed costs, low survival rate and high feed conversion ratio. The purpose of this acivity was to solve the problems faced by the farmers of catfish culture. The method of this activity was to transfer a knowledge and appropriate technology to farmers through Participatory Rural Appraisal and Participatory Technology Development. The activities that have been carried out in catfish culture are: (1) socialization, training and mentoring of probiotic production, (2) socialization, training and mentoring of fermented feed production using soy flour and wheat bran (3) construction of 2 m diameter of tarpaulin tanks , (4) provision of catfish seeds 7 cm, (5) the application of fermented feed and probiotic, and (6) monitoring and evaluation.
LIMBAH BANDENG (Chanos chanos Forsskal) SEBAGAI SUMBER PROTEIN PENGGANTI TEPUNG IKAN DALAM PAKAN UDANG GALAH (Macrobracium rosenbergii de Man) Arning Wilujeng Ekawati; Muhammad Fakhri; Joko Abdillah; Wiwit Nor Indahsari
Journal of Aquaculture and Fish Health Vol. 8 No. 3 (2019): JAFH vol. 8 no. 3 September 2019
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.36 KB) | DOI: 10.20473/jafh.v8i3.15048

Abstract

Tujuan penelitian ini untuk menentukan jumlah terbaik pemanfaatan tepung limbah bandeng (Chanos chanos) sebagai sumber protein pengganti tepung ikan dalam pakan udang galah (M. rosenbergii de Man). Penelitian ini  menggunakan  Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Pakan yang digunakan sebagai perlakuan adalah pakan iso protein 35% dan iso energi 3,40 kkal/g dengan perbedaan subtitusi protein tepung limbah bandeng terhadap tepung ikan, yaitu: (A) 0%; (B) 25%; (C) 50%; (D) 75%; dan (E) 100%. Parameter yang diamati dan dianalisis terdiri dari:  kelulushidupan, laju pertumbuhan spesifik, rasio konversi pakan, rasio efisiensi protein, dan retensi protein pada udang galah (M. rosenbergii de Man). Hasil penelitian menunjukkan bahwa pemanfaatan tepung limbah bandeng sebagai sumber protein hewani pengganti tepung ikan dengan persentase yang berbeda dalam formula pakan tidak berpengaruh nyata terhadap semua parameter yang diamati pada udang galah (M. rosenbergii de Man). Berdasarkan hasil penelitian dapat disimpulkan bahwa pemanfaatan tepung limbah bandeng dapat menggantikan tepung ikan sebagai sumber protein dalam pakan udang galah (M. rosenbergii de Man) hingga 100%.
Growth, Pigment and Protein Production of Spirulina platensis under different Ca(NO3)2 concentrations Muhammad Fakhri; Prive Widya Antika; Arning Wilujeng Ekawati; Nasrullah Bai Arifin
Journal of Aquaculture and Fish Health Vol. 9 No. 1 (2020): JAFH Vol. 9 no. 1 February 2020
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.922 KB) | DOI: 10.20473/jafh.v9i1.15769

Abstract

Spirulina platensis is a filamentous cyanobacterium that has been commerically used for fish feed and human food supplement. Low-cost production of Spirulina is needed when considering large-scale culture especially for industrial purposes. The aim of this study was to explain the effect of calcium nitrate (Ca(NO3)2) on growth, biomass, pigment, and protein production of S. platensis and to determine the best calcium nitrate concentration for Spirulina production.The microalgae was cultured at four calcium nitrate concentrations (1, 1.5, 2.0 and 2.5 g/L) with salinity of 15 ppt, constant light intensity of 4,000 lux and photoperiod of 24:0 light:dark cycles for 4 days. The results showed that different calcium nitrate concentrations remarkably affected the growth, biomass production, pigment and protein content of S. platensis (p<0.05). The highest specific growth rate of 0.721 day-1 and biomass concentration of 1.512 g/Lwere achieved at calcium nitrate concentration of 2.5 g/L. Moreover, the algae had the highest chlorophyll-a, carotenoid and protein content at 2.5 g L-1. Increasing calcium nitrate concentration from 1 to 2.5 g/L led to an increase in biomass, pigment and protein production of S. platensis. However, there was no significant difference between 2 and 2.5 g L-1 calcium nitrate concentrations. We suggest that 2-2.5 g/L Ca(NO3)2 concentration can be used profitably for S. platensis production.
Diatomae Chaetoceros ceratosporum dalam Formula Pakan Meningkatkan Respon Imun Seluler Udang Windu (Penaeus monodon Fab.) Arning Wilujeng Ekawati; Happy Nursyam; Edi Widjayanto; Marsoedi Marsoedi
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1049.923 KB) | DOI: 10.21776/ub.jels.2012.002.01.04

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh dan dosis yang terbaik pada pemanfaatan diatomae Chaetoceros ceratosporum dalam formula pakan terhadap peningkatan respon imun seluler udang windu (Penaeus monodon Fab.). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Sebagai perlakuan adalah pemanfaatan Chaetoceros ceratosporum dalam formula pakan iso protein 39,02% dan iso energy 3,58 kkal g pakan dengan dosis yang berbeda yaitu: A (0%), B (3,04%), C (6,08%) dan D (9,12%). Parameter yang diamati adalah total hemosit, total diferensial hemosit (hyaline, semi granular dan granular), dan aktivitas vibriocidal. Berdasarkan hasil penelitian menunjukkan bahwa pemanfaatan diatomae Chaetoceros ceratosporum dalam formula pakan dapat meningkatkan respon imun seluler, dan dosis terbaik berkisar 5,15% - 6,51%. Kata kunci: Chaetoceros ceratosporum, respon imun seluler, udang windu (Penaeus monodon Fab.)
The Use of Full-Grained, Fragmented, and Reduced Marine Yeast Powder in Fodder Formulation to Improve the Growth of Eel Fish (Anguilla bicolor) Ria Retno Dewi Sartika Manik; Arning Wilujeng Ekawati; Hardoko Hardoko
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.126 KB) | DOI: 10.21776/ub.jels.2016.006.02.05

Abstract

Ikan Sidat or eel fish (Anguilla bicolor) is a popular fish commodity in domestic trade as well as for export commodity. The use of marine yeast as fodder suffers from some weaknesses since it contains nucleic acids and rigid thick cell walls that obstruct the absorbance of nutrition. Optimizing the use of marine yeast as fodder ingredients can be done by fragmenting and reducing the yeast. This research attempts at optimizing the use of marine yeast regarding its rate of growth, fodder efficiency and the survival rate of eel fish. This research employs the complete random design using 4 different treatments and 3 repetition. In the four treatments, the fodder contained protein (45%) and iso energy (3.600 kkal.kg-1 fodder), which were applied to the treatment A 0% (control), B 5% (complete), C 5% (fragmented) and D 5% (reduced). The parameters used in this study were survival rate, specific growth rate, ratio of fodder conversion, ratio of protein efficiency, energy retention, protein retention and rate of metabolism. The data of this study showed the best result in the treatment D, 5% (reduced), showing survival rate value of 95.56%, specific growth at 1.12, food conversion ratio of 2.27, protein efficiency ratio of 0.98, protein retention at 18.26%, energy retention of 16.57, and protein metabolism rate at 81.96%. Keywords: eel fish, growth, marine yeast.