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Istiyanto Samidjan
Lecturer Department of aquaculture, fisheries and Marine Science Faculty of Diponegoro University Jl. Prof. Sudharto,SH, Kampus Tembalang, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Engineering Technology Fish Farming of Snakeheads Channa striat ,Bloch, 1793) Based Feed Vitamin C Increase to Superior Quality Using Microsatellite Istiyanto Samidjan; Diana Rachmawati
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.66 KB) | DOI: 10.20884/1.oa.2018.14.2.571

Abstract

Objectives for snakeheads farming Cngineered to produce superior products based artificial diet eenrichment vitamin C with different doses and the use of microsatellites to increase growth and determine the superior quality. Methods February to November 2017 Examined the Effect of artificial feed Enriched with vitamin C doses per 100 g feed were given 0 mg (T1), 12 mg (T2), 24 mg ,and 36 mg (T3), 48 mg (T4) were using a completely randomized design (CRD) and 4 treatments and 3 replications. Furthermore, to determine the superior quality using microsatellite genetic markers to see the genetic code snakeheads. Then the treatment was analyzed by ANOVA and data arrangement of molecular weight DNA, polymorphism were analyzed by Cco-stat and Minitab version 11. The data collected observations of the size of the  weight absolute, survival, FCR,  molecular weight DNA composition, heterogeneity genotype, polymorphism. The results showed that highest of polymorphism were T3 (weight absolute 169.18 mg) and lowest ppolymorphism  treatment T1 (weight absolute 149.25 mg) .Next analysis of polymorphism results using a micro-satellite in treatment (T2, ribbon 3,4), and (T3, ribbons 5.6) and (T4, ribbons 7 and 8)  respectively using IS-GB1F 5-CCC TGT ATT TCA TTT CTC CA-3 and IS-GB 1 R 5-ACC AAC ACT GCA ATC TCT CT-3 bright ribbons found in treatment T4 (ribbons 7.8) snakeheads are the results showed that the growth of snakeheads were highest in T3 (with ribbons 205bp allele ladder with 215 bps and 225 bps on the right, Addition as well as having polymorphism is high and. variation of different types also found the number of alleles and heterozygotes.
Polyculture Engineering of White Shrimp Vannamei and Seaweed on Different Planting Distance on The Growth, Survival in Abration Pond Istiyanto Samidjan; Diana Rachmawati
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.822 KB) | DOI: 10.20884/1.oa.2018.14.2.569

Abstract

The objective of the study was to assess the role of polyculture engineering of vanamei shrimp, and seaweed in an effort to increase growth and survival. The experimental method was randomized complete design with 4 treatments and 3 replications were T1 (10 PL / m2 white shrimp vannamei and seaweed distance 15 cm), T2 (10 PL / m2 of white shrimp vannamei and seaweed distance 30 cm), T3 (10 PL / m2 white shrimp vanamei and seaweed distance  45 cm, T4 (10 PL / m2 white shrimp vanamei and seaweed 60 cm). Seaweed used Gracillaria sp type 150 g / bundle with treatment used a long line system placed around the nets plot of 1 m2 in pond culture 300 m2. Data includes the growth of absolute weight, survival, FCR were analyzed variety and test Tukey. observation of the water quality of maintenance media include nitrite, ammonia, temperature, pH, salinity using descriptive. There is a very real effect (P <0.01) on treatment with different plant spacing on seaweed and vanamei shrimp on growth and survival of white shrimp vanamei and seaweed. The highest absolute weight growth in vanamei shrimp was T4 treatment (vanamei shrimp 29.25 ± 0.07 g), and seaweed (2919.03 ± 8.70bg) of white shrimp vanamei survival rate (98.25% ± 2.25%) and FCR (food Conversion ratio) 1.15 ± 0.09. Water quality is still feasible optimum.
Co-Authors Diana Rachmawati