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All Journal BIOTROPIC The Journal of Tropical Biology Jurnal Labora Medika
Yeni Avidhatul Husnah
Stikes Prima Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology

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Profil Senyawa Bioaktif Ekstrak Kalus Biji Jeruk Purut (Citrus hystrix DC.) Pasca Induksi Metil Jasmonat Yeni Avidhatul Husnah; Ari Indrianto; Woro A.S Tunjung
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (854.475 KB) | DOI: 10.29080/biotropic.2019.3.2.146-160

Abstract

Kaffir lime (Citrus hystrix DC.) is an alternative source of potential and relatively safe anticancer compounds as it contains bioactive components. Previous study has shown that the extract of kaffir lime leaves contains terpenoids and potentially as anticancer. However, the content of bioactive compounds may vary due to environmental factors. Callus culture is an alternative method of induction of bioactive compound content with a controlled environment. Previous study has succeeded in inducing callus from kaffir lime seed explants produce the ideal callus with a crumb / friable texture. Methyl jasmonate is reported to induce the production of secondary metabolite compounds. Elicitation with methyl jasmonate is one of the strategy to increase terpenoids from cultured cells Objective of this study is anlyze the using of methyl jasmonate as elicitor.to increase bioactive compounds inkaffir lime cell suspension. Callus induced from seed explants age 40 days were transferred into liquid medium for the initiation of suspension culture. Subculture is done when the age of culture reaches 21 days then measured thegrowth curve o f cell supension and concentration of sucrose in medium. After cells reached log phse in 12 days then be treated with methyl jasmonic for 8 days. The cell suspension then beextracted with ethyl acetate by maceration method. The content of bioactive compounds in the extract was anlyzed with GC-MS. Suspension of lime lime cells C. hystrix, without treatment yields fatty acid compounds. Bioactive compounds produced by suspended cell suspension after methyl jasmonate elicitation is germacrene A, myrcene, alpha-terpineol, geranyl acetate, trans-caryophyllene, delta-guaiene and several types of ester fatty acids.
REACTION AGGLUTINATION OF SESAME SEED EXTRACT (Sesamum indicum L.) TEST IN BLOOD GROUP O yeni avidhatul husnah
Jurnal Labora Medika Vol 3, No 1 (2019): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2578.502 KB) | DOI: 10.26714/jlabmed.3.1.2019.23-28

Abstract

Blood type O has no antigens blood cells, but can produce antibodies against antigens A and B. Detection of blood group O antigen is currently based on the presence of H. Examination with anti-H was found from seed lectin Ulex, Ulex while living only in subtropical areas. Sesame is a member of Family Pedaliaceae thrives in the tropics. This study aims to obtain anti-O derived from biological sources Indonesia and determine the effect on a sesame seed extract agglutination reaction with blood group O. The research was conducted by the method of manufacture of dry sesame seed extract that has been done dilution with solvent concentration ratio is 1:2; 1:10, and 1:100 supernatant were subsequently taken. In making Ulex seed extract with a concentration of 1:10, further testing on the blood group of each seed extract concentration. Based on the research that has been done, the result that sesame seed extract is able to lyse erythrocytes in the blood group A, B, and O concentration ratio for all treatments and did not reveal any agglutination reaction in all treatments. Meanwhile, the Ulex europaeus seed extract can mengagglutinasi erythrocytes in blood type O. It can be concluded that the extract of sesame seeds can not be used as an anti-H replaces Ulex seeds, sesame seeds but potentially as an agent of hemolysis. 
Co-Authors Ari Indrianto Woro A.S Tunjung