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All Journal Jurnal Matematika & Sains Indonesian Journal of Biotechnology
Erly Marwani
Departemen Biologi, FMIPA ITB-Bandung
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Mathematics

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Isolasi Enterobacteriaceae Patogen dari Makanan Berbumbu dan Tidak Berbumbu Kunyit (Curcuma longa L.) Serta Uji Pengaruh Ekstrak Kunyit (Curcuma longa L.) Terhadap Pertumbuhan Bakteri Yang Diisolasi Ernin Hidayati; Nuryati Juli; Erly Marwani
Jurnal Matematika & Sains Vol 7, No 2 (2002)
Publisher : Institut Teknologi Bandung

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An isolation of pathogenic Enterobacteriaceae bacteria has been done on spiced foods with turmeric and without turmeric ingredients. Food samples were taken randomly from 8 traditional food vendors at a traditional market in north Bandung during three seasons for 6 months. Next step was extraction of turmeric rhizome with sequential extraction method using n-hexane, ethyl acetate and ethanol. Those extracs were applied onto 4 chosen bacterial isolates using Kirby Bauer method, cylinder/hole plate method, and dilution method. There were 8 bacterial isolates which always present in all season. Also there were 4 isolates only found in rainy season. Eight isolates among them were pathogens. Total frequency of bacteria in samples with turmeric ingredients was lower than that without turmeric ingredients. The effect of extracts on all tested bacteria (Escherchia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus), showed that n-hexane extract could inhibit the growth of Escherchia coli, Klebsiella pneumoniae and Staphylococcus aureus at concentration of 50.000 ppm on solid medium. However in liquid medium, all tested bacteria were inhibited at the concentration of 1000 ppm.
Peningkatan Produksi Azadirahtin dalam Kultur Suspensi Sel Azadirachta indica A.Juss melalui Penambahan Skualen Zulfa Zakiah; Erly Marwani; Arbayah H. Siregar
Jurnal Matematika & Sains Vol 8, No 4 (2003)
Publisher : Institut Teknologi Bandung

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One of the methods to enhance the secondary metabolite content in plant tissue culture is precursor feeding. The study on azadirachtin enhancement in cell suspension culture of Azadirachta indica A.Juss was conducted by supplying squalene as precursor. Callus were induced on Murashige-Skoog solid medium with addition of 0,5μM 2,4-dichlorophenoxy acetic acid (2,4-D) and 1,0μM 6-benzylaminopurine (BAP). Callus were subcultured to Murashige-Skoog liquid medium with addition of 0,1μM 2,4-D and 1,0μM BAP. The cell suspension cultures were subcultured three times, and then treated each with 10, 100, and 1000μM of squalene. Azadirachtin content was measured at 0, 2, 4, 6, 8, 10 and 12 days after feeding using HPLC (High Pressure Liquid Chromatography) with methanol:water (6:4) as mobile phase. The results showed that the highest azadirachtin content in cell suspension culture before supplying squalene was 0,041 g/g cell dry-weight (dw) at the age of 8 days culture. The increment of azadirachtin content in cell and medium was significantly influenced by squalene concentration and age of culture. The highest azadirachtin content after feeding in the cell mass (0,076 ± 0,006 g/g dw) and in the medium (0,229 ± 0,003 mg/L)was achieved on supplying 100μM squalene to 10 and 12 days culture.
Agrobacterium-Mediated Stable Transformation of Medicinal Plant Andrographis paniculata Callus Expressing β-glucuronidase (GUS) Gene Erly Marwani; Agustina Tangapo; Fenny Martha Dwivany
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (406.629 KB) | DOI: 10.22146/ijbiotech.7873

Abstract

This study was carried out to establish a stable genetic transformation in callus culture of Andrographispaniculata mediated by Agrobacterium tumefaciens. The leaf disks of A. paniculata were infected with A. tumefaciensLBA4404 carrying a binary vector pCAMBIA1304 that contain β-glucuronidase (GUS) and hygromycinphosphotransferase (hpt) genes. The infection was conducted by dipping method for one hour, followed byco-cultivation in the dark for three days. To examine transient GUS expression, the co-cultivated leaf disks wereassayed for β-glucuronidase activity and to obtain stable transformed callus, the co-cultivated leaf disks wereselected on the callus induction medium which contain 20 mg/l hygromycin for selection. The transformedcallus was periodically subcultured every three weeks into the fresh selection medium over the 15 weeksperiod. To test a stable transformation, the callus was subjected to PCR analysis for GUS gene detection. Theresults indicated that the co-cultivated leaf disks expressed GUS activity and proliferated to produce callus onthe selective medium. Analysis of PCR on the transformed callus indicated the presence 976 bp fragment thatconfi rmed the presence of β-glucuronidase gene. These fi ndings imply that the β-glucuronidase was stably integrated into A. paniculata callus culture.Keywords: Andrographis paniculata, Agrobacterium tumefaciens, andrographollide, transformed callus,β-glucuronidase gene.
Co-Authors Agustina Tangapo Arbayah H. Siregar Ernin Hidayati FENNY MARTHA DWIVANY Nuryati Juli Zulfa Zakiah