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All Journal Jurnal Ilmiah Farmasi Pharmacology and Clinical Pharmacy Research
Madyawati Latief
University of Jambi
Medicine & Pharmacology

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Isolation of alkaloids compound of ethanol extract of mangrove perepat (S. alba) root and its antibacterial activity Madyawati Latief; Muhaimin Muhaimin; Hilda Amanda; Indra Lasmana Tarigan; Siti Aisyah
Jurnal Ilmiah Farmasi Vol. 17 No. 1 (2021): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol17.iss1.art2

Abstract

 Abstract  Background: Exploration of the chemical contents and bioactivities of mangrove plants is important to discover new therapeutic agents for society and to develop its medicinal values.Objective: This study aims to isolate alkaloid compounds from the ethanol extract of Sonneratia alba root which is likely to have an antibacterial activity.Methods: Isolation was carried out using fractionation and chromatography, while antibacterial activity test was performed using the disc diffusion methods.Results: Our phytochemical screening shows that the ethanol extract has a high concentration of alkaloid compounds. The alkaloid which contain in isolate (F4) is further confirmed through UV-Vis Spectra and FT-IR spectra. The ethanol extract exhibits the antibacterial activity against S.  aureus and E.  coli with the inhibition zone of 1.5 mm and 2.5 mm, respectively.Conclusion: Alkaloid compound has been isolated from Sonneratia alba and has potential as antibacterial agent.Keywords: mangrove perepat (Sonneratia alba), alkaloid, antibacterial agent Intisari Latar Belakang: Eksplorasi kandungan kimiawi dan bioaktivitas tanaman mangrove penting dilakukan untuk menemukan agen terapeutik baru bagi masyarakat serta mengembangkan nilai medisnya.Tujuan: Penelitian ini bertujuan untuk mengisolasi senyawa alkaloid dari ekstrak etanol akar Sonneratia alba yang berpotensi menghasilkan aktivitas antibakteri.Metode: Isolasi senyawa dilakukan dengan fraksinasi dan kromatografi, sedangkan antibakteri dilakukan dengan metode difusi cakram.Hasil: Hasil skrining fitokimia yang telah dilakukan menunjukkan bahwa ekstrak etanol memiliki kandungan  alkaloid yang tinggi. Kandungan alkaloid dalam Isolat (F4) telah dikonfirmasi secara spektrofotometri UV-Vis dan FT-IR. Aktivitas antibakteri ekstrak etanol terhadap S.  aureus dan E.  coli ditunjukkan dengan dihasilkannya zona hambat sebesar 1,5 mm dan 2,5 mm.Kesimpulan: Senyawa alkaloid berhasil diisolasi dari Sonneratia alba serta memiliki potensi sebagai agen antibakteri.Kata Kunci: mangrove perepat (Sonneratia alba), alkaloid, antibakteri
Potential Tracking OF Cytotoxic Activities Of Mangrove Perepate (Sonneratia alba) Root Extract as an Anticancer Candidate Madyawati Latief; Nelson Nelson; Hilda Amanda; Indra Lasmana Tarigan; Siti Aisyah
Pharmacology and Clinical Pharmacy Research Vol 5, No 2
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.145 KB) | DOI: 10.15416/pcpr.v5i2.26790

Abstract

Mangrove plants are one of the most extensive biodiversity in Jambi. Besides functioning as a coastal ecosystem protector, it also has any potential of its chemical content. The study of mangrove’s bioactive compound is important to generate a new therapeutic agent. This study aims to obtain chemical compounds that have cytotoxic activity, reveal the chemical formula and molecular structure of that active compound, and determine the cytotoxic activity level of active compound from perepat (Sonneratia alba) root extract. The extract was isolated and purified using Liquid Vacuum Chromatography, Graphitation Column Chromatography, and Thin Layer Chromatography. The isolate was characterized using FT-IR spectrophotometry. Our result found that it corresponds to the β-sitosterol compound group with a high similarly chromophore. Furthermore, both of the extract and isolate was tested for toxicity activity using the Brine Shrimp Lethality Test (BSLT) method using Artemia salina shrimp larvae. Herein, we reported that acetone isolated extract possesses lower cell death average 100% (start 200 μg/ml) than isolate extract 80% (50μg/ml). Furthermore the toxicity of total extract has LC50 23,98 (μg/ml), while Isolate 10,04 (μg/ml). From the LC50 value, it can be concluded that the toxicity of the extract lower than the isolate, and both were classified as very toxic compounds, and very potential to be developed into anti-cancer drug compounds.
Antidiabetic Activity of Sungkai (Peronema canescens Jack) Leaves Ethanol Extract on the Male Mice Induced Alloxan Monohydrate Madyawati Latief; Putri M. Sari; Liddini T. Fatwa; Indra L. Tarigan; H. P. Vasantha Rupasinghe
Pharmacology and Clinical Pharmacy Research Vol 6, No 2 (2021)
Publisher : Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15416/pcpr.v6i2.31666

Abstract

  Sungkai (Peronema canescens Jack) is an alternative natural ingredient that can be used as an antidiabetic containing several secondary metabolites which are thought to reduce blood glucose levels by inhibiting the action of the α-glucokinase enzyme and increasing the activity of antioxidant enzymes and being able to regenerate β-cells, damaged pancreas so that insulin deficiency can be overcome. The aim of this study was to determine the effect of P. canescens leaves ethanol extract on antidiabetic activity and to determine the effect of various doses of sungkai leaf ethanol extract as antidiabetic. The scientific research method used in this study was a completely randomized design (CRD) and data analysis used in this study is the one way ANOVA, following by the Duncan test. Blood glucose measurement in treated mice was carried out 3 times. Mice were devided into five groups, three mice in each group, and each group was administered different dosages of sungkai extract (T1 = extract 175 mg/kg body weight; T2 = extract 350 mg/kg body weight; T3 = extract 700 mg/ kg body weight). First, the mice were acclimatized for six days and the injection of treatment was started at day 7. Blood glucose levels, urine volume, body weight and daily drinking of mice was carried out before induction (day 0), after induction (day 8) and after the end of treatment for 10 days (day 18), was carried out using the Point of Care Test (POCT) method using the Easy Touch GCU. The results showed that the ethanol extract of sungkai leaves had the potential to reduce blood glucose levels in mice. The ethanol extract of sungkai leaves at a dose of 350 mg/kg body weight has the most optimal potential to reduce blood glucose levels, urine volume, daily drinking, and body weight. 
Co-Authors H. P. Vasantha Rupasinghe Hilda Amanda Indra L. Tarigan Indra Lasmana Tarigan Liddini T. Fatwa Muhaimin Muhaimin Nelson Nelson Putri M. Sari Siti Aisyah