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All Journal JURNAL KIMIA SAINS DAN APLIKASI Jurnal Riset Kimia
Zolkapli Eshak
Faculty of Pharmacy, Universiti Teknologi MARA, Selangor Darul Ehsan, Malaysia
Chemical Engineering, Chemistry & Bioengineering Chemistry Engineering

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CHARACTERISTIC OF APOPTOSIS AND EXPRESSION OF GENES-RELATED APOPTOSIS (c-myc, c-erb AND c-fos ONCOGENE) IN HeLa CELL LINES AFTER EXPOSURE BY NEEM (Azadirachta indica A.Juss) Arisanty, Dessy; Eshak, Zolkapli; Othman, Fauziah; Rahmat, Asmah; Akim, Abdah M.D.; -, Nurmawaty; M. Jalil, Suhaida.
Jurnal Riset Kimia Vol 1, No 2 (2008): March
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v1i2.35

Abstract

 ABSTRACT Azadirachta indica A. Juss is a medicinal plant commonly known as neem. The effect of neem leaves extract on cervical cancer cells, however, has never been studied. Due to the lack of information, this study was conducted to determine the effect of neem leaves extract on cervical cancer (HeLa) cell growth. In vitro cytotoxicity effect of ethanolic neem extract indicated the presence of cytotoxicity activity of the extract against HeLa cells with IC50 of  30.0 μg/mL. The morphological changes under confocal laser scanning microscope (CLSM) on HeLa cells were cell shrinkage and membrane blebbing. There were also cells with condensed nucleus and few cells have fragmented nucleus, and finally formed apoptotic body. Control cells showed a clear cytoplasm and centrally placed nucleus and no cells exhibited any apoptotic features. Appearance of apoptotic cells under scanning electron microscope (SEM) are indentations, blebs and hole on cell surface and disintegration of cell. The controls remained morphologically normal. Apoptotic features of the cells are widely seen with longer incubation time while 24 hours incubation time, it is scarcely seen. The RT-PCR product showed that the c-erb gene expression was expressed in both treated and untreated HeLa cells. Contrary, the the c-myc and c-fos oncogenes on  HeLa cells which exposed to A. indica  EtOH extract were significantly decreased.  Thus, the results from this study strongly suggest that the ethanolic extract of A. indica may contain bioactive compound(s) that caused cervical cancer cells, HeLa cell death by apoptosis mechanism and lead to succession of discovering new alternative treatment for cervical cancer.  Keywords : cytotoxic, apoptosis genes, oncogenes, neem
The Effect of miR-10b Mimic Transfection on Invasion and Proliferation Gene Expression as the Direct Target of the miR-10b Pathway in T47D Breast Cancer Cell Lines Arisanty, Dessy; Eshak, Zolkapli; Wahyuni, Fatma Sri; Achyar, Afifatul; Khairani, Salsabila Putri
Jurnal Kimia Sains dan Aplikasi Vol 29, No 1 (2026): Volume 29 Issue 1 Year 2026
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jksa.29.1.58-63

Abstract

Breast cancer remains one of the leading causes of death in women worldwide, with high incidence and mortality rates. The abnormal expression of the small non-coding RNA, microRNA-10b (miR-10b), has been associated with breast cancer progression. miR-10b is involved in various biological processes, including proliferation, metastasis, and drug resistance. However, the underlying mechanism by which miR-10b contributes to breast cancer progression remains unclear. This study aimed to investigate the effect of miR-10b on the expression of HOXD10 and PI3K genes, as well as its impact on cell viability and proliferation in breast cancer cells. In this study, T47D breast cancer cells were transfected with mimic miR-10b using the FANA-oligo transfection method at concentrations of 1, 2.5, and 5 µM. Cell viability was assessed using the MTT assay, while HOXD10 and PI3K gene expression levels were measured using RT-qPCR and ELISA. Data were analysed using paired t-tests with SPSS, and results were considered statistically significant at p < 0.05. The results showed that mimic miR-10b induced a dose-dependent increase in cell proliferation. Notably, only HOXD10 gene expression was significantly affected by the transfection, while PI3K gene expression remained unchanged. Cells transfected with 5 µM miR-10b mimic exhibited the highest proliferation rate compared to non-transfected controls (p < 0.05). In conclusion, miR-10b promotes breast cancer cell proliferation by downregulating HOXD10 expression, independent of PI3K, suggesting it is a potential target for further investigation.
Co-Authors Abdah M.D. Akim Achyar, Afifatul Asmah Rahmat Dessy Arisanty Fatma Sri Wahyuni Fauziah Othman Khairani, Salsabila Putri Nurmawaty - Suhaida. M. Jalil