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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kimia Terapan Indonesia
Euis Filailla
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Materials Science & Nanotechnology

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Effect of Type and Concentration of Encapsulating Agents on Physicochemical, Phytochemical, and Antioxidant Properties of Red Dragon Fruit Kombucha Powdered Beverage Rena Agatha; Yati Maryati; Agustine Susilowati; Aspiyanto Aspiyanto; Anastasia Fitria Devi; Hani Mulyani; Setyani Budiari; Euis Filailla; Della Rahmawati; Nina Artanti
Jurnal Kimia Terapan Indonesia Vol 23, No 1 (2021)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/inajac.v23i1.474

Abstract

Kombucha is a healthy beverage from the fermentation of sugared tea with a symbiotic culture of bacteria and yeast (SCOBY). There is a growing interest on kombucha due to the reported health benefits. The original kombucha is prepared using only sweetened black tea infusion. The development of kombucha, however, has been reported to incorporate other plant water extracts. A previous study showed that kombucha beverage using red dragon fruit (RDF) as an alternative substrate gave the highest antioxidant activity at 15 days of fermentation. RDF kombucha instant powder could be a new food product development due to its longer shelf life, convenience, and low distribution cost. This study aimed to determine the best and the concentration of encapsulating agent for RDF kombucha powdered beverage which could retain the antioxidant properties. RDF kombucha was spray dried using maltodextrin, gum arabic, and inulin as the encapsulating agents with various concentrations (5%, 10%, and 15%). Gum arabic was the best encapsulating agent since it gave the highest values of drying yield, pH, and  total phenolic content retention, in comparison to the other two encapsulating agents. Within the observed concentrations, the incorporation of gum arabic resulted in RDF kombucha powder with a similar appearance, red colour, and antioxidant retention. Thus, the 5% addition of gum arabic is recommended for future preparations of RDF kombucha powdered beverage.
Effect of Liquid Media Composition on Alpha-Glucosidase Inhibitory Activity from Aspergillus elegans SweF9 Hani Mulyani; Rizna Triana Dewi; Euis Filailla; Nina Artanti
Jurnal Kimia Terapan Indonesia Vol 21, No 2 (2020)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jkti.v21i2.426

Abstract

This study aims to determine the effect of liquid media composition on the inhibitory activity of α-glucosidase from endophytic fungus Aspergillus elegans SweF9 isolated from seaweed (Macroalgae Euchema sp). Fermentation was carried out in three types of liquid media, namely: potato dextrose broth (PDB), potato malt peptone (PMP), and Czapek-dox broth (CDB), which was incubated for 10 days at room temperature, with static conditions. Ethyl acetate solvents were used to extract active metabolites from fungal biomass and filtrate from each media. Antidiabetic activity was measured based on inhibition of enzyme α-glucosidase. The results showed that filtrate extract of A. elegans SweF9 which was cultured on the media PDB showed highest inhibitor activity to the α-glucosidase enzyme with an IC50 value of 1.74 µg / mL compared to other extracts. Based on these results, the PDB media is an appropriate medium for culturing A. elegans SweF9  to produces secondary metabolites that can be used as a new source of antidiabetic agents.
Antidiabetic Activity of Cocor Bebek Leaves (Kalanchoe pinnata Lam.Pers.) Ethanolic Extract from Various Areas Indah Dwiatmi Dewiyanti; Euis Filailla; Megawati Megawati; Tri Yuliani
Journal of Tropical Life Science Vol. 2 No. 2 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Antidiabetic activity of Cocor Bebek leaves (Kalanchoe pinnata Lam.Pers.) ethanolic extract from Bogor city, Kabupaten Bogor and south of Tangerang city has been studied. The study was conducted in vitro using glucosidase inhibitor method. The results of the study showed that IC50 of the extract from Bogor city, Kabupaten Bogor, and Tangerang Selatan city is 40.94 ppm, 33.58 ppm and 16.12 ppm respectively. Meanwhile, IC50 of quersetin which has antidiabetic activity is 10.22 ppm. The results showed that Cocor Bebek leaves (Kalanchoe pinnata Lam.Pers.) ethanolic extract had antidiabetic activity with IC50 less than 100 ppm. However, the activity is lesser than quercetin.Keywords:Kalanchoe pinnata Lam.Pers, antidiabetic activity, glucosidase inhibitor
Co-Authors Agustine Susilowati Anastasia Fitria Devi Aspiyanto Aspiyanto Della Rahmawati Hani Mulyani Hani Mulyani Indah Dwiatmi Dewiyanti Megawati Megawati Nina Artanti Nina Artanti Rena Agatha Rizna Triana Dewi Setyani Budiari Tri Yuliani Yati Maryati