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Anthonius Y.P.B.C. Widyatmoko
Center for Forest Biotechnolog y and Tree Improvement Research
Agriculture, Biological Sciences & Forestry

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STUDY ON GENETIC VARIATION AND RELATIONSHIPS AMONG FOUR ACACIA SPECIES USING RAPD AND SSCP MARKER Anthonius Y.P.B.C. Widyatmoko; Atsushi Watanabe; Susumu Shiraishi
Indonesian Journal of Forestry Research Vol 7, No 2 (2010): Journal of Forestry Research
Publisher : Secretariat of Agency for Standardization of Environment and Forestry Instruments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2010.7.2.125-143

Abstract

Genetic diversity and relationship of four Acacia species, Acacia aulacocarpa, A. Cunn. ex Benth., A. auriculiformis Cunn. ex Benth., A. crassicarpa Cunn. ex Benth. and A. mangium Willd. were investigated using DNA molecular markers. Using RAPD analysis, a total of 20 arbitrar y primers successfully gave 127 polymorphic fragments. These RAPD data were used to estimate genetic distance and construct dendrograms using the unweighted pair-group with the arithmetic mean average (UPGMA) method. The four species were divided into two major clusters. A. auriculiformis and A. mangium were in one cluster, and the other cluster contained both A. aulacocarpa and A. crassicarpa. The divergence time of the two species (A. auriculiformis and A. mangium) in the former cluster appeared to be relatively early in comparison to in the latter (A. aulacocarpa and A. crassicarpa) based on RAPD data. This result was also supported by Principal Component Analysis (PCA). Among the four species, A. aulacocarpa showed the highest divergence in nuclear DNA (ncDNA), followed by A. auriculiformis. SSCP analysis also revealed that these two species possessed a haplotypic variation of the trnL-trnF intergenic spacer region of chloroplast DNA. In A. aulacocarpa, a large difference in the composition of both nuclear and chloroplast genomes was obser ved between populations distributed in Queensland, Australia and those in New Guinea Island. In contrast, compared to the other species, A. mangium showed the lowest genetic diversity and less genetic differentiation among populations.
SEQUENCE POLYMORPHISMS OF FOUR CHLOROPLAST GENES IN FOUR ACACIA SPECIES Anthonius Y.P.B.C. Widyatmoko; Susumu Shiraishi
Indonesian Journal of Forestry Research Vol 8, No 1 (2011): Journal of Forestry Research
Publisher : Secretariat of Agency for Standardization of Environment and Forestry Instruments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2011.8.1.79-89

Abstract

Sequence polymorphisms among and within four Acacia species,  A. aulacocarpa, A. auriculiformis, A. crassicarpa, and A. mangium, were investigated using four chloroplast DNA genes (atpA, petA, rbcL, and rpoA). The phylogenetic relationship among these species is discussed in light of the results of the sequence information. No intraspecific sequence variation was found in the four genes of the four species, and a conservative rate of mutation of the chloroplast DNA genes was also confirmed in the Acacia species. In the atpA and petA of the four genes, all four species possessed identical sequences, and no sequence variation was found among the four Acacia species. In the rbcL and rpoA genes, however, sequence polymorphisms were revealed among these species. Acacia aulacocarpa and A. crassicarpa shared an identical sequence, and A. auriculiformis and A. mangium also showed no sequence variation.  The fact that A. mangium and A. auriculiformis shared identical sequences as did A. aulacocarpa and A. crassicarpa indicated that the two respective species were extremely closely related. Although a putative natural hybrid of A. aulacocarpa and A. auriculiformis has been reported, our results suggested that natural hybridization should be further verified using molecular markers.
Co-Authors Atsushi Watanabe Susumu Shiraishi