F.X. Susilo
Bidang Proteksi Tanaman Jurusan Agroteknologi, Fakultas Pertanian, Universitas Lampung Jl. Prof. Sumantri Brojonegoro No. 1 Bandar Lampung 35145

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PERTUMBUHAN DAN PERKEMBANGAN IN VITRO JAMUR Beauveria bassiana MUTAN SERTA VIRULENSINYA TERHADAP HAMA PENGISAP POLONG KEDELAI (Riptortus linearis) DI LABORATORIUM Lita Aprianda Sari; F.X. Susilo; Yuyun Fitriana; Lestari Wibowo
Jurnal Agrotek Tropika Vol 7, No 3 (2019): JAT September 2019
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (991.413 KB) | DOI: 10.23960/jat.v7i3.3550

Abstract

Penelitian ini bertujuan mengetahui pertumbuhan dan perkembangan in vitro jamur Beauveria bassiana mutan serta virulensinya terhadap hama pengisap polong kedelai, R. linearis. Penelitian dilakukan di LaboratoriumBioteknologi Pertanian Fakultas Pertanian Universitas Lampung dan dilaksanakan bulan Januari - Juni 2017. Uji pertumbuhan B. bassiana secara in vitro menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 5 ulangan. Uji virulensi jamur B. bassiana terhadap R. linearis menggunakan analisis probit. Virulensi diindikasikan dengan LT 50 atau lethal time 50, yaitu waktu yang dibutuhkan jamur ini untuk mematikan 50% larva uji. Hasil penelitian menunjukkan bahwa isolat Bbyf22 dan Bbyf24 (mutan) mampu tumbuh dan berkembangdengan normal seperti isolat Bbyf (wildtype). Jamur B. bassiana terbukti virulen terhadap hama R. linearis dengan LT 50 = 3,7 hari (isolat Bbyf22, mutan); 4,9 hari (isolat Bbyf24, mutan); dan 3,5 hari (isolat Bbyf, wildtype).
KELIMPAHAN DAN KERAGAMAN NEMATODA PADA PERTANAMAN BAWANG MERAH (Allium cepa L.) DI LAMPUNG TENGAH Adela Maria Vianni; I Gede Swibawa; F.X. Susilo; Titik Nur Aeny; Ni Kadek Emi Sintha Dewi
JURNAL AGROTROPIKA Vol. 25 No. 2 (2026): Jurnal Agrotropika Vol 25 No 2, Oktober 2026
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v25i2.13301

Abstract

Shallot (Allium cepa L.) is an important horticultural crop whose productivity is influenced by nematodes. This study aimed to identify and analyze the abundance and diversity of nematode communities in shallot plantations in Central Lampung. The research was conducted from October 2025 to February 2026. Nematode samples were collected using a systematic zigzag sampling method in shallot plantations in Rejo Asri Village, Seputih Raman District, Central Lampung Regency. Samples were taken from three habitats: shallot plants, shallot plantation soil, and fallow soil near the shallot plantations. Nematodes were extracted from soil using decantation, sieving, and sugar-solution centrifugation methods, while extraction from plant tissue utilized a modified Baermann funnel method. Nematodes were identified to the genus level based on morphological characteristics. Community analysis included Prominence Value (PV), Margalef index (DMg), Shannon index (H’), evenness index (E), Simpson’s index (H₂), Bray–Curtis dissimilarity index, Jaccard similarity index, and the Michaelis–Menten model. The results revealed the presence of 28 nematode genera, comprising 13 plant-parasitic genera and 15 free-living genera. Diversity indices for the habitats were as follows: shallot plantation soil (S = 21, DMg = 3.16, H' = 2.32, H₂ = 0.87), fallow soil (S = 20, DMg = 2.75, H' = 2.48, H₂ = 0.90), and shallot plants (S = 10, DMg = 1.46, H' = 1.33, H₂ = 0.59). The maximum number of genera predicted by the Michaelis–Menten model was 29. The two habitats exhibiting the highest community similarity were shallot plants and shallot plantation soil, with a Jaccard Similarity (JS) of 40.9%. The dominant plant-parasitic nematode genera are Aphelenchoides, with an absolute density of 2.5 individuals per plant and a PV of 22.3, and Helicotylenchus, with an absolute density of 72.8 individuals per 300 cc of soil and a PV of 727.5.