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All Journal Jurnal Biologi Tropis Seminar Nasional Peningkatan Mutu Pendidikan
Ari Hepi Yanti
Universitas Tanjungpura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Social Sciences

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Aktivitas Enzimatik Streptomyces spp. Yang Diisolasi Dari Usus Dan Feses Cacing Nipah (Namalycastis rhodochorde) Rikhsan Kurniatuhadi; Ari Hepi Yanti; Tri Rima Setyawati
SEMINAR NASIONAL PENINGKATAN MUTU PENDIDIKAN Vol. 1 No. 1 (2020): Seminar Nasional Peningkatan Mutu Pendidikan
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Samudra

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Abstract

Streptomyces terlibat dalam proses degradasi senyawa organik pada substrat pakan cacing nipah (Namalycastis rhodochorde). Streptomyces mensekresikan beberapa enzim untuk memecah makromolekul organik menjadi senyawa sederhana yang tersedia bagi detritivor cacing nipah. Penelitian ini bertujuan untuk mendeteksi aktivitas enzim enam Streptomyces spp. yang telah diisolasi sebelumnya dari usus dan feses cacing nipah. Aktivitas enzim dideteksi pada empat media yang berbeda: CMC-congo red agar, skim milk agar, gelatin nutritive agar, dan starch agar. Aktivitas katalase dideteksi menggunakan 3% larutan H2O2. Hasil penelitian menunjukkan bahwa enam Streptomyces spp. memiliki aktivitas selulolitik, amilolitik, pencairan gelatin, dan katalase. Namun hanya dua dari enam isolate Streptomyces spp yang memiliki aktivitas protease, yaitu Streptomyces NrAFA2 dan Streptomyces NrAFA4. Streptomyces NrAFA4 memiliki nilai indeks aktivitas proteolitik dan selulolitik tertinggi, yaitu 0,54 dan 1.0. sedangkan nilai indeks aktivitas selulolitik tertinggi terjadi pada isolat Steptomyces NrAFA3 yaitu 1.57. Profil aktivitas enzimatik Streptomyces spp. Yang diisolasi dari usus dan feses cacing nipah dapat dimanfaatkan untuk pengembangan formula pakan untuk budidaya cacing nipah.
Pathogenicity Characteristics of Front, Middle, and Hind Nipah Worm (Namalycastis rhodochorde) Digestive Tract Bacteria Dian Indrawati; Ari Hepi Yanti; Rikhsan Kurniatuhadi
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12427

Abstract

Nipah worms (Namalycastis rhodochorde) are one of Indonesia's invertebrate species used as high-protein aquaculture feed. The presence of pathogenic bacteria is a factor in the decline in quality and quantity in nipah worm cultivation, especially bacteria originating from the digestive tract of nipah worms. This study was conducted to examine the characteristics of pathogenicity of bacteria originating from the digestive tract of Nipah worms in the form of hemolysis and DNase activity. The hemolysis and DNase activity tests were performed using the streak plate method on Blood Agar Plate (BAP) and DNase Agar media. The results obtained in the hemolysis test showed a positive reaction in 68 isolates (90.7%), consisting of 5 α-hemolysis isolates, 63 β-hemolysis isolates, and 7 γ-hemolysis isolates. β hemolysis or complete hemolysis was found in 27 isolates in the foregut, 18 isolates in the midgut, and 18 isolates in the hindgut. The results obtained in the DNase activity test were positive in 17 isolates (22.7%), consisting of 9 isolates from the front digestive tract, 1 isolate from the middle digestive tract, and 7 isolates from the rear digestive tract. This study concludes that the high levels of hemolysis and DNase activity in bacterial isolates from the digestive tract of the nipah worm are predictors of pathogenicity. Bacterial isolates from the foregut showed the highest pathogenic activity, which is thought to be due to the presence of bacteria carried during the feeding process of the nipah worm.
Co-Authors Dian Indrawati Rikhsan Kurniatuhadi, Rikhsan Tri Rima Setyawati