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All Journal Jurnal Penelitian Hutan Tanaman Jurnal Entomologi Indonesia
Teguh Santoso
Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management

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FUNGI PADA BATANG POHON Paraserianthes falcataria DAN ASOSIASINYA DENGAN Xystrocera festiva (Coleoptera : Cerambycidae) Yunasfi Yunasfi; Soetrisno Hadi; Gayuh Rahayu; Teguh Santoso
Jurnal Penelitian Hutan Tanaman Vol 6, No 4 (2009): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1931.21 KB) | DOI: 10.20886/jpht.2009.6.4.251-259

Abstract

Penelitian ini bertujuan untuk mengetahui jenis-jenis fungi yang berkembang pada batang pohon Paraserianthes falcataria yang diserang oleh Xystrocera festiva. Fungi diisolasi dari kulit dan kayu gubal pada beberapa tahap gerekan X. festiva. Acremonium sp. 1 berhasil diisolasi dari kulit batang. Pada permukaan kayu gubal ditemukan Trichoderma virens dan Acremonium sp. (aff. Acremonium sp. st. imperfect Nectria arenuloides). Trichoderma harzianum dan Acremonium sp. (aff. Acremonium sp. st. imperfect N. cyathea) ditemukan pada terowongan kayu gubal yang dibuat oleh X. festiva. Acremonium spp. juga terdapat pada larva dan imago X. festiva. Hal ini menunjukkan adanya interaksi antara Acremonium spp. dengan X. festiva. Terdapat indikasi bahwa T. virens dan T. harzianum menekan perkembangan Acremonium spp. pada batang pohon P. Falcataria.
Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) Farriza Diyasti; Teguh Santoso; R. Yayi Munara Kusumah
Jurnal Entomologi Indonesia Vol 13 No 3 (2016): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.92 KB) | DOI: 10.5994/jei.13.3.117

Abstract

Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) is one of the most important pests on cotton. Yield losses due to this pest can reach up to 70%. Pest control using the NPV has been proven to be effective in the field. However NPV isolates from different geographical locations could be showed variation in genetic and their biology. The purpose of this study was to compare morphology, biology, and molecular characteristics between three HearNPV isolates from cotton field in South Sulawesi, Central Java, and East Java. Scanning electron microscope (SEM) was used to look at morphological characters, whereas biological characters were observed by dipping shelled of young corn cob on each isolate suspension with a density of 2.5 x 103, 2.5 x 104, 2.5 x 105, 2.5 x 106, 2.5 x 107 POBs /ml and control then fed to the first instar larvae of H. armigera. Probit analysis was used to determine LC and LT. The molecular assay used polymerase chain reaction (PCR) with lef-8 gene. The results showed that the three isolates had the shape and size of polyhedra which was not significantly different with the mean diameter were 0.86 μm. The result showed that in 7 days post infection, HearNPV isolate from East Java was the most virulent isolates with the lowest LC50, which is 6.38 POBs/ml, but lowest LT50 value in 2.5 x 107 POBs/ml were showed both from East Java and South Sulawesi, which are 2.7 days post infection. Meanwhile, the DNA sequencing of the three isolates showed a high similarity (100% homology).
Co-Authors Farriza Diyasti Gayuh Rahayu Kusumah, Yayi Munara Soetrisno Hadi Yunasfi Yunasfi