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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Yelnititis Yelnititis
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta
Agriculture, Biological Sciences & Forestry

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INDUKSI EMBRIO SOMATIK Shorea pinanga Scheff. PADA KONDISI FISIK MEDIA BERBEDA Yelnititis Yelnititis
Jurnal Pemuliaan Tanaman Hutan Vol 7, No 2 (2013): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2013.7.2.73-84

Abstract

Shorea pinanga (Scheff.) is a member of Dipterocarpaceae which has an important role both as a forest timber and also non forest timber product (tengkawang). The purpose of this experiment is to obtain the best treatment for somatic embryos formation. Friable callus was used as explants whereas liquid and solid MS media supplemented with vitamin B, 30 g/l sucrose were used as basal medium. Embryogenic callus was induced through three times subcultured in basal medium with 5.0 mg/l 2,4- D. Somatic embryo was induced using 100 mg of embryogenic callus with the addition of 0.5, 1.0, and 1.5 mg/l kinetin. The observation was done on texture, numbers and color of embryogenic callus and weekly number of somatic embryos for eight weeks. The kinetin treatment of 1.5 mg/l on liquid medium was the best treatment because it is able to induce 162 cotyledonary stage of somatic embryos for eight weeks.
Mikropropagasi Ramin (Gonytilus bancanus(Miq) Kurz) dari Eksplan Batang satu buku secara in Vitro Yelnititis Yelnititis; Tadjudin Edy Komar
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 3 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.3.149-157

Abstract

Ramin (Gonystylus spp.) is one of the genus wood producing that interested to trade. This plant growth in peat swamp forest areas. This genus consist more than 20 species and only Gonystylus bacanus (Miq.) Kurz) has been explored. From 2004 this species has been listed in CITES APPENDIX II. Propagation of this plant can conducted trhrough generative but difficult to get of materials as explants. Murashige and Skoog (MS) medium used as growth medium. The observation was made  on Shoot induction period, shoot number, heigh of shoot and visual performance of culture produced. The results showed that shoot can induced from stem with single node. The treatment of 2.0 mg/l thidiazuron is the best treatment to shoot induction. The average of shoot find was 5.1 days after cultured  and faster than other treatment. The treatment of 0.5 mg/l BA is the best to shoot growth and visual performance. Average of shoot height from this treatment is 0.7 cm. The treatment of 0.5 mg/l BA on modifild MS medium is the best to shoot elongation than MS medium.
PERBANYAKAN TUNAS Gyrinops versteegii (Gilg.) Domke Yelnititis Yelnititis
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 2 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.2.108-120

Abstract

Gyrinops versteegii (Gilg.) Domke) is a member of Thymellaceae having an important role as agarwood source that is potential to develop. Agarwood is a non timber forest product (NTFP) with a high economic value. Gyrinops versteegii (Gilg) Domke was an endemic and rare plants, however, it is cultivated on the island of  Lombok. The study of shoot multiplication from single node stem and cotyledon node explants to shoot  produced was conducted to find out the best method to shoot multiplication. Modification of Murashige and Skoog (MS), Gamborg (B5) dan Woody Plant Medium (WPM) supplemented with 8.0 g/l agar, 30 g/l sucrose and vitamin (0.1 mg/l Thyamine, 0.5 mg/l Nicotinic acid, 0.5 mg/l Pyridoxine dan 2 mg/l Glysin) and 10 g/l myoinositol were used as growth medium treatments. The experiment was conducted on two stages i.e. germination and shoot multiplication. Shoot induction was conducted on modification of MS medium supplemented with Benzyl Adenin (BA) 0.5; 1.0 and1.5 mg/l. The multiplication of shoot conducted on  modification of MS medium, WPM and B5 medium supplemented with 0.5; 0.75 dan 1.0 mg/l Benzyl Adenin (BA). For every treatment there were ten samples (bottles) with one explants in each bottle. The observation was conducted on shoot induction percentage, number of shoot and visual performance of culture. The result showed that modified MS + BA 0.5 mg/l was the best for shoot induction from single node stem explants with the average of 1.6 shoots on 12 weeks. The treatment of modified MS + BA 0.75 mg/l was the best for shoot multiplication from single node stem explants with the average of 5.7 shoots on 12 weeks. The treatment of modified WPM + 0.5 mg/l BA was the best for shoot multiplication from cotyledonery node explants with the average of 4.6 shoots on 12 weeks. The visual performance of shoot resulted was normal.
Co-Authors Tadjudin Edy Komar