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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
Yelnititis Yelnititis
Centre for Forest Biotechnology and Tree Improvement Research and Development
Agriculture, Biological Sciences & Forestry

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INDUKSI KALUS EMBRIOGENIK DAN EMBRIO SOMATIK DARI EKSPLAN DAUN KULIM (Scorodacarpus borneensis Becc.) Yelnititis Yelnititis
Jurnal Pemuliaan Tanaman Hutan Vol 14, No 2 (2020): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2020.14.2.73-81

Abstract

Kulim is one of woody plant that have multifunction as wood source and for spice and medicinal. Generative propagation of this plant have trouble because seed use limited. The use of leaf segment through somatic embryogenesis to solve the problem. The objective of this study is to obtain the best treatment to embryogenic callus induction. The modification of basal medium of Murashige and Skoog was used as growth medium. The experiment was conducted in three stages are callus induction, embryogenic callus and somatic embryo induction. The treatment of 2,4-D (3,0 – 12 mg/l) used for callus induction. For embriogenic callus induction used 2,4-D (3,0 – 12,0 mg/l) combined with NAA 0,5 mg/l. The treatment of thidiazuron (0,1 – 0,7 mg/l) used for somatic embryo induction. The result showed that the treatment of 2,4-D 6,0 mg/l is the best for callus induction with compact of texture, green, dry and non embryogenic. The treatment of combination 2,4-D 12.0 mg/l with NAA 0.5 mg/l is the best for friable callus induction. The treatment of 2,4-D 6.0 mg/l combined with NAA 0,5 mg/l is the best for embryogenic callus induction with very friable of texture, easy to separate, dry, smooth and glossy. Thidiazuron of 0,1 mg/l treatment is the best for somatic embryos induction with the average number of 7,8 somatic embryos.
PEMBENTUKAN EMBRIO SOMATIK DARI EKSPLAN DAUN RAMIN, SPESIES TANAMAN LANGKA Yelnititis Yelnititis
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.129-135

Abstract

Ramin (Gonystylus bancanus (Miq.) Kurz) is one of the most valuable timbers in Indonesia and the most over-exploited woody species, on the other hand, the success in propagation techniques are still limited. This species has been listed in Appendix II of CITES since 2004 as the number of trees and populations continuously decline. Tissue culture has been explored for mass propagation, however, this technique still faces the challenge, mainly in shoot elongation regenerated from single node explants.  The purpose  of  these experiments is  to select the best auxin (2,4-D, picloram, dan dicamba) and the best concentration of Benzyl Adenine (BA)  treatment on  somatic embryo  formation. A series of experiments of somatic embryogenesis from leaf explant for ramin were conducted at Tissue Culture Laboratory, Center for Forest Biotechnology and Tree Improvement Yogyakarta. Modified Murashige and Skoog (MS) media supplemented with 0,1 mg/L thiamine; 0,5 mg/l nicotinic acid; 0,5 mg/L pyridoxine; 2.0 mg/L glycine and 100 mg/L myo-inositol were used as growth medium. In this study, three different auxins were used as treatments: 2,4-D (2,4 dichlorophenoxy acetic acid), picloram, and dicamba applied at 6.0 mg/L. The observation was made on the texture of callus formed and the performance of the somatic embryos obtained. The results showed that the texture of callus obtained is compact formed callus and green in color. The best treatment to induce globular somatic embryos is using 6.0 mg/L picloram within eight months. The best treatment to induce globular and torpedo somatic embryos is BA 3.0 mg/L treatment.
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