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All Journal JURNAL PERBENIHAN TANAMAN HUTAN
Y.M.M Anita Nugraheni
BP2TPTH
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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Hormones Effect on Gyrinops versteegii (Gilg.) Domke Shoots Cutting using Water Rooting Method Y.M.M Anita Nugraheni; Kurniawati Purwaka Putri
Jurnal Perbenihan Tanaman Hutan Vol 6, No 2 (2018): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.594 KB) | DOI: 10.20886/bptpth.2018.6.2.85-92

Abstract

Gyrinops versteegii is one of protected species because of the rare existence. One of the efforts to keep the preserved is by cultivation. Cultivation techniques using generative basis has already done a lot. However, this technique has several limitations, due to the recalcitrant character of the seeds and the inequality of the characteristics of the progeny with their parent. Meanwhile, vegetative techniques will produce a new individual that have the same characteristics as the parent. The alternative-promising vegetative techniques of G. versteegii are using media because it is cheap and easy to obtain, and increase the achievement if additional hormones were used. The purpose of this research is to determine the optimum concentration of the NAA hormone for G. versteegii cuttings. The research method is done by cutting the saplings of G. versteegii on the shoots. The medium used are water without hormones (as control), water with hormones of NAA 100, 200, and 300 ppm. Observations 10 times, at the end of the observation carried out measurements on the root, percent of life, buds, dry stem weight, and dry root weight. The result showed that NAA hormone treatment influenced significantly on the growth of the roots, percent of life, dry stem weight and dry weight of roots of G. versteegii. Medium of water with the addition of NAA 300 ppm was the composition that can be used as a medium to trigger initial rooting.
Pre Sterilization Effect towards the Successful of the Initiation of Sengon Explant Shoots Y.M.M Anita Nugraheni; Ratna Uli Damayanti Sianturi; Yulianti Bramasto
Jurnal Perbenihan Tanaman Hutan Vol 7, No 1 (2019): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (543.776 KB) | DOI: 10.20886/bptpth.2019.7.1.45-54

Abstract

Sengon known as forest product with high market demand. Tissue culture is one multiplication method to get seeds in large quantities. One factors that support the success of reproduction is the health of the plant material. The purpose of the study is to determine the influence of pre-sterilization treatments towards the successful initiation of sengon organogenesis. The research method was done by spraying the seedlings with Pyraclostrobin 50 g.kg -1+ Metiram 550 g.kg -1of 2,500 ppm every week for 3 months. Each explant was grown on MS medium.The observation was carried out done every 1 week for 12 weeks, including the amount of contamination (bacteria, fungi), browning and the appearance of buds, roots and callus. Data were analyzed by t-test. The next stage is multiplication by using treatments of 24D 0.1 mM, 24D 0.5 mM, BAP 0.5 mM, BAP 2 mM, K 0.1 mM, K 0.5 mM, TDZ 0.1 mM, TDZ 0, 5 mM, and control. Completely randomized design was used and analyzed by using one way ANOVA, and tested with Duncan for the differences at 0,05 significant level. The results showed that pra-sterilization treatment was capable of supporting the successful initiation of sengon shoots characterized by low levels of contamination, increased number of callus and number of sengon shoots explants. Kinetin 0.01 mM in the multiplication media of sengon shoots was able to increase the number of adventitious shoots.
Co-Authors Kurniawati Purwaka Putri Ratna Uli Damayanti Sianturi Yulianti Bramasto