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Asriani Ilyas Asriani Ilyas
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Chemistry

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Isolasi dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n-Heksan dari Umbi Lobak (Raphanus Sativus Lamk) Ummi Zahra; Muharram Muharram; Asriani Ilyas Asriani Ilyas
Al-Kimia Vol 1 No 1 (2013): June
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (545.813 KB) | DOI: 10.24252/al-kimia.v1i1.1570

Abstract

Isolation and identification of secondary metabolite compound in extract of n-Hexane in Umbi Lobak (Raphanus sativus Lamk) have been carried out. The purpose of this research is to know secondary metabolite compound which is contained in Umbi Lobak (Raphanus sativus Lamk) which obtained from Gowa. The compound was obtained by isolation process that consists of several steps including extraction, fractination, purification, and identification. Extraction was carried out using maceration with n- hexane. The identification has done by phytochemistry test, melting point, solubility, TLC, and IR spectroscopy. The result of research found out that the obtained compound was the steroid compound, with melt point 133-133,5 oC and had positiveĀ  reaction toward Lieberman Burchad reagents, where the Lieberman Burchad reagent resulting is green which perfectly solved in n-hexane, bit solved in methanol, and could not be solved in aceton. TLC analysis showed a stain in three scales different solvent and the result of elucidation structure in IR spectrophotometer showed that compound which get is Ī² sitosterol.
Co-Authors Muharram Muharram Ummi Zahra, Ummi