Article Per Year (5 Year)
p-Index From 2021 - 2026
0
P-Index
This Author published in this journals
All Journal Al-Kimia
Edin Ningsih
Balai penelitian Tanaman Rempah dan Obat, Jalan Tentara Pelajar No. 3, Bogor Barat 16111
Chemistry

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Extraction, Isolation, Characterisation and Antioxidant Activity Assay of Catechin Gambir (Uncaria gambir (Hunter). Roxb Edin Ningsih; Sri Rahayuningsih
Al-Kimia Vol 7 No 2 (2019): DECEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.394 KB) | DOI: 10.24252/al-kimia.v7i2.7800

Abstract

Catechins are one of polyphenol compounds which reported have antioxidant properties. This study aims to extraction, isolation, characterization, and determine the antioxidant activity of catechin compounds from gambier powder(Uncaria gambir (Hunter). The extraction method used was maceration extraction with three types of solvents (70% ethanol, 70% methanol, and ethyl acetate). The process of purifying catechins is carried out with water, ethyl acetat, and n-hexane. Catechins were characterized by FTIR (Fourier Transform Infra Red) and UV-Vis spectrophotometer and tested for antioxidant activity using the DPPH (2.2 Difenyl -1-Pikrilhidrazil) method. The results showed that the extracted gambier using 70% ethanol solvent resulted in more catechins (51.20 g) than the 70% methanol solvent (40.35 g) and ethyl acetate (16.40 g). Characterization of catechin using UV-Vis spectrophotometer showed that purity content of catechins obtained from the extract ethanol 70% had purity  70.76%, methanol 70% 70.86%, and ethyl acetate 70.35%. Analysis using FTIR showed catechin has functional groups such as hydroxy (-OH), vibration C = C alkene, C-H, CH2 and C-O, so that the compound isolated has the basic structure of the catechin. The result of antioxidant activity from showed IC50 value are 2.72 ppm; 3.04 ppm and 3.06 ppm for extract catechin of etanol 70%, methanol 70% and ethyl acetate, so that can be said that catechins have antioxidant activity levels in a very strong category.
Co-Authors Sri Rahayuningsih